The relationship between physical and genetic distances at the Hor1 and Hor2 loci of barley estimated by two-colour fluorescent in situ hybridization
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The relationship between physical and genetic distances at the Hor1 and Hor2 loci of barley estimated by two-colour fluorescent in situ hybridization. / Pedersen, C.; Linde-Laursen, I.
I: Theoretical and Applied Genetics, Bind 91, Nr. 6-7, 01.11.1995, s. 941-946.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The relationship between physical and genetic distances at the Hor1 and Hor2 loci of barley estimated by two-colour fluorescent in situ hybridization
AU - Pedersen, C.
AU - Linde-Laursen, I.
PY - 1995/11/1
Y1 - 1995/11/1
N2 - The hordeins are the major class of storage proteins in barley. They are encoded by multigene families. The B- and C-hordein loci have been mapped physically to the distal end of chromosome 5 (1I) of cultivated barley by fluorescent in situ hybridization. Based on measurements of chromosomal distances between the two hordein loci, the relationship between genetic and physical distances has been estimated to be about 1 mega base pairs per centiMorgan. This is four times higher than the mean value for the barley genome as a whole and confirms the tendency to increased recombination in distal chromosome regions. The resolving power of two-colour FISH is discussed. It is concluded that the method is suitable for estimating the relationship between genetic and physical distances of regions of about 10 Mbp or larger.
AB - The hordeins are the major class of storage proteins in barley. They are encoded by multigene families. The B- and C-hordein loci have been mapped physically to the distal end of chromosome 5 (1I) of cultivated barley by fluorescent in situ hybridization. Based on measurements of chromosomal distances between the two hordein loci, the relationship between genetic and physical distances has been estimated to be about 1 mega base pairs per centiMorgan. This is four times higher than the mean value for the barley genome as a whole and confirms the tendency to increased recombination in distal chromosome regions. The resolving power of two-colour FISH is discussed. It is concluded that the method is suitable for estimating the relationship between genetic and physical distances of regions of about 10 Mbp or larger.
KW - Hordeins
KW - Hordeum vulgare
KW - In situ hybridization
KW - Physical and genetic chromosome maps
KW - Recombination
UR - http://www.scopus.com/inward/record.url?scp=0001016305&partnerID=8YFLogxK
U2 - 10.1007/BF00223904
DO - 10.1007/BF00223904
M3 - Journal article
AN - SCOPUS:0001016305
VL - 91
SP - 941
EP - 946
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 6-7
ER -
ID: 226915442