This paper reports the results of a translation and validation of a Danish language version of the IPIP-NEO-120 constructed by Johnson. The IPIP-NEO-120 is a public domain 120-item inventory that measures personality constructs similar to those in the Revised NEO Personality Inventory. The inventory was developed to provide researchers with a free inventory with a shorter format than the earlier 300-item IPIP-NEO and hence enable more researchers to include a personality measure in their research. The validation study reported in this paper was conducted to expand the IPIP-NEO-120 into the Danish context and provide Danish researchers with a free, validated personality measure based on the Five-Factor Model directly applicable to cross-national research. The psychometric properties of the Danish IPIP-NEO-120 were tested in a sample of veterinary students and graduates (N = 525) from a Danish university. The factor structure, internal consistency, and factor intercorrelations obtained in the Danish sample closely resembled those obtained in Johnson’s validation of the original IPIP-NEO-120, and the results indicate that the Danish measure corresponds well to the English language original.