TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium

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TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium. / Clement, Christian Alexandro; Ajbro, Katrine Dalsgaard; Koefoed, Karen; Vestergaard, Maj Linea; Veland, Iben Rønn; Perestrello Ramos H de Jesus, Maria; Pedersen, Lotte Bang; Benmerah, Alexandre; Andersen, Claus Yding; Larsen, Lars Allan; Christensen, Søren Tvorup.

I: Cell Reports, Bind 3, Nr. 6, 2013, s. 1806-1814.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Clement, CA, Ajbro, KD, Koefoed, K, Vestergaard, ML, Veland, IR, Perestrello Ramos H de Jesus, M, Pedersen, LB, Benmerah, A, Andersen, CY, Larsen, LA & Christensen, ST 2013, 'TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium', Cell Reports, bind 3, nr. 6, s. 1806-1814. https://doi.org/10.1016/j.celrep.2013.05.020

APA

Clement, C. A., Ajbro, K. D., Koefoed, K., Vestergaard, M. L., Veland, I. R., Perestrello Ramos H de Jesus, M., Pedersen, L. B., Benmerah, A., Andersen, C. Y., Larsen, L. A., & Christensen, S. T. (2013). TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium. Cell Reports, 3(6), 1806-1814. https://doi.org/10.1016/j.celrep.2013.05.020

Vancouver

Clement CA, Ajbro KD, Koefoed K, Vestergaard ML, Veland IR, Perestrello Ramos H de Jesus M o.a. TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium. Cell Reports. 2013;3(6):1806-1814. https://doi.org/10.1016/j.celrep.2013.05.020

Author

Clement, Christian Alexandro ; Ajbro, Katrine Dalsgaard ; Koefoed, Karen ; Vestergaard, Maj Linea ; Veland, Iben Rønn ; Perestrello Ramos H de Jesus, Maria ; Pedersen, Lotte Bang ; Benmerah, Alexandre ; Andersen, Claus Yding ; Larsen, Lars Allan ; Christensen, Søren Tvorup. / TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium. I: Cell Reports. 2013 ; Bind 3, Nr. 6. s. 1806-1814.

Bibtex

@article{32d0f6b85dbd43b995914d797d49884c,

title = "TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium",

abstract = "Transforming growth factor β (TGF-β) signaling is regulated by clathrin-dependent endocytosis (CDE) for the control of cellular processes during development and in tissue homeostasis. The primary cilium coordinates several signaling pathways, and the pocket surrounding the base and proximal part of the cilium is a site for CDE. We report here that TGF-β receptors localize to the ciliary tip and endocytic vesicles at the ciliary base in fibroblasts and that TGF-β stimulation increases receptor localization and activation of SMAD2/3 and ERK1/2 at the ciliary base. Inhibition of CDE reduced TGF-β-mediated signaling at the cilium, and TGF-β signaling and CDE activity are reduced at stunted primary cilia in Tg737(orpk) fibroblasts. Similarly, TGF-β signaling during cardiomyogenesis correlated with accumulation of TGF-β receptors and activation of SMAD2/3 at the ciliary base. Our results indicate that the primary cilium regulates TGF-β signaling and that the ciliary pocket is a compartment for CDE-dependent regulation of signal transduction. VIDEO ABSTRACT:",

author = "Clement, {Christian Alexandro} and Ajbro, {Katrine Dalsgaard} and Karen Koefoed and Vestergaard, {Maj Linea} and Veland, {Iben R{\o}nn} and {Perestrello Ramos H de Jesus}, Maria and Pedersen, {Lotte Bang} and Alexandre Benmerah and Andersen, {Claus Yding} and Larsen, {Lars Allan} and Christensen, {S{\o}ren Tvorup}",

year = "2013",

doi = "10.1016/j.celrep.2013.05.020",

language = "English",

volume = "3",

pages = "1806--1814",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - TGF-β Signaling Is Associated with Endocytosis at the Pocket Region of the Primary Cilium

AU - Clement, Christian Alexandro

AU - Ajbro, Katrine Dalsgaard

AU - Koefoed, Karen

AU - Vestergaard, Maj Linea

AU - Veland, Iben Rønn

AU - Perestrello Ramos H de Jesus, Maria

AU - Pedersen, Lotte Bang

AU - Benmerah, Alexandre

AU - Andersen, Claus Yding

AU - Larsen, Lars Allan

AU - Christensen, Søren Tvorup

PY - 2013

Y1 - 2013

N2 - Transforming growth factor β (TGF-β) signaling is regulated by clathrin-dependent endocytosis (CDE) for the control of cellular processes during development and in tissue homeostasis. The primary cilium coordinates several signaling pathways, and the pocket surrounding the base and proximal part of the cilium is a site for CDE. We report here that TGF-β receptors localize to the ciliary tip and endocytic vesicles at the ciliary base in fibroblasts and that TGF-β stimulation increases receptor localization and activation of SMAD2/3 and ERK1/2 at the ciliary base. Inhibition of CDE reduced TGF-β-mediated signaling at the cilium, and TGF-β signaling and CDE activity are reduced at stunted primary cilia in Tg737(orpk) fibroblasts. Similarly, TGF-β signaling during cardiomyogenesis correlated with accumulation of TGF-β receptors and activation of SMAD2/3 at the ciliary base. Our results indicate that the primary cilium regulates TGF-β signaling and that the ciliary pocket is a compartment for CDE-dependent regulation of signal transduction. VIDEO ABSTRACT:

AB - Transforming growth factor β (TGF-β) signaling is regulated by clathrin-dependent endocytosis (CDE) for the control of cellular processes during development and in tissue homeostasis. The primary cilium coordinates several signaling pathways, and the pocket surrounding the base and proximal part of the cilium is a site for CDE. We report here that TGF-β receptors localize to the ciliary tip and endocytic vesicles at the ciliary base in fibroblasts and that TGF-β stimulation increases receptor localization and activation of SMAD2/3 and ERK1/2 at the ciliary base. Inhibition of CDE reduced TGF-β-mediated signaling at the cilium, and TGF-β signaling and CDE activity are reduced at stunted primary cilia in Tg737(orpk) fibroblasts. Similarly, TGF-β signaling during cardiomyogenesis correlated with accumulation of TGF-β receptors and activation of SMAD2/3 at the ciliary base. Our results indicate that the primary cilium regulates TGF-β signaling and that the ciliary pocket is a compartment for CDE-dependent regulation of signal transduction. VIDEO ABSTRACT:

U2 - 10.1016/j.celrep.2013.05.020

DO - 10.1016/j.celrep.2013.05.020

M3 - Journal article

C2 - 23746451

VL - 3

SP - 1806

EP - 1814

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 6

ER -

ID: 46232043