Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii

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Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii. / Wubshet, Sileshi Gizachew; Nyberg, Nils; Tejesvi, Mysore V.; Pirttilä, Anna Maria; Kajula, Marena; Mattila, Sampo; Stærk, Dan.

I: Journal of Chromatography A, Bind 1302, 2013, s. 34-39.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Wubshet, SG, Nyberg, N, Tejesvi, MV, Pirttilä, AM, Kajula, M, Mattila, S & Stærk, D 2013, 'Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii', Journal of Chromatography A, bind 1302, s. 34-39. https://doi.org/10.1016/j.chroma.2013.05.032

APA

Wubshet, S. G., Nyberg, N., Tejesvi, M. V., Pirttilä, A. M., Kajula, M., Mattila, S., & Stærk, D. (2013). Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii. Journal of Chromatography A, 1302, 34-39. https://doi.org/10.1016/j.chroma.2013.05.032

Vancouver

Wubshet SG, Nyberg N, Tejesvi MV, Pirttilä AM, Kajula M, Mattila S o.a. Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii. Journal of Chromatography A. 2013;1302:34-39. https://doi.org/10.1016/j.chroma.2013.05.032

Author

Wubshet, Sileshi Gizachew ; Nyberg, Nils ; Tejesvi, Mysore V. ; Pirttilä, Anna Maria ; Kajula, Marena ; Mattila, Sampo ; Stærk, Dan. / Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii. I: Journal of Chromatography A. 2013 ; Bind 1302. s. 34-39.

Bibtex

@article{7319cc5f831b4e758ce8779a4716efa9,
title = "Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii",
abstract = "The high-resolution radical scavenging profile of an extract of the endophytic fungus Penicillium namyslowskii was used to target analysis by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy, i.e., HPLC-HRMS-SPE-NMR, for identification of anti-oxidative secondary metabolites. This revealed the two chromatographic peaks with the highest relative response in the radical scavenging profile to be griseophenone C and peniprequinolone. The HPLC-HRMS-SPE-NMR analysis was performed in the tube-transfer mode using a cryogenically cooled NMR probe designed for 1.7-mm NMR tubes. To further explore the potential of the above HPLC-HRMS-SPE-NMR platform for analysis of endophytic extracts, six peaks displaying no radical scavenging activity were also analyzed. This allowed unambiguous identification of six metabolites, i.e., dechlorogriseofulvin, dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, mevastatin acid, and mevastatin. The high mass sensitivity of the 1.7-mm cryogenically cooled NMR probe allowed for the first time acquisition of direct detected 13C NMR spectra of fungal metabolites, i.e., dechlorogriseofulvin and griseofulvin, directly from crude extract via HPLC-HRMS-SPE-NMR. Dechlorodehydrogriseofulvin was reported for the first time from nature.",
author = "Wubshet, {Sileshi Gizachew} and Nils Nyberg and Tejesvi, {Mysore V.} and Pirttil{\"a}, {Anna Maria} and Marena Kajula and Sampo Mattila and Dan St{\ae}rk",
year = "2013",
doi = "10.1016/j.chroma.2013.05.032",
language = "English",
volume = "1302",
pages = "34--39",
journal = "Journal of Chromatography",
issn = "0301-4770",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Targeting high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance analysis with high-resolution radical scavenging profiles - bioactive secondary metabolites from the endophytic fungus Penicillium namyslowskii

AU - Wubshet, Sileshi Gizachew

AU - Nyberg, Nils

AU - Tejesvi, Mysore V.

AU - Pirttilä, Anna Maria

AU - Kajula, Marena

AU - Mattila, Sampo

AU - Stærk, Dan

PY - 2013

Y1 - 2013

N2 - The high-resolution radical scavenging profile of an extract of the endophytic fungus Penicillium namyslowskii was used to target analysis by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy, i.e., HPLC-HRMS-SPE-NMR, for identification of anti-oxidative secondary metabolites. This revealed the two chromatographic peaks with the highest relative response in the radical scavenging profile to be griseophenone C and peniprequinolone. The HPLC-HRMS-SPE-NMR analysis was performed in the tube-transfer mode using a cryogenically cooled NMR probe designed for 1.7-mm NMR tubes. To further explore the potential of the above HPLC-HRMS-SPE-NMR platform for analysis of endophytic extracts, six peaks displaying no radical scavenging activity were also analyzed. This allowed unambiguous identification of six metabolites, i.e., dechlorogriseofulvin, dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, mevastatin acid, and mevastatin. The high mass sensitivity of the 1.7-mm cryogenically cooled NMR probe allowed for the first time acquisition of direct detected 13C NMR spectra of fungal metabolites, i.e., dechlorogriseofulvin and griseofulvin, directly from crude extract via HPLC-HRMS-SPE-NMR. Dechlorodehydrogriseofulvin was reported for the first time from nature.

AB - The high-resolution radical scavenging profile of an extract of the endophytic fungus Penicillium namyslowskii was used to target analysis by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy, i.e., HPLC-HRMS-SPE-NMR, for identification of anti-oxidative secondary metabolites. This revealed the two chromatographic peaks with the highest relative response in the radical scavenging profile to be griseophenone C and peniprequinolone. The HPLC-HRMS-SPE-NMR analysis was performed in the tube-transfer mode using a cryogenically cooled NMR probe designed for 1.7-mm NMR tubes. To further explore the potential of the above HPLC-HRMS-SPE-NMR platform for analysis of endophytic extracts, six peaks displaying no radical scavenging activity were also analyzed. This allowed unambiguous identification of six metabolites, i.e., dechlorogriseofulvin, dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, mevastatin acid, and mevastatin. The high mass sensitivity of the 1.7-mm cryogenically cooled NMR probe allowed for the first time acquisition of direct detected 13C NMR spectra of fungal metabolites, i.e., dechlorogriseofulvin and griseofulvin, directly from crude extract via HPLC-HRMS-SPE-NMR. Dechlorodehydrogriseofulvin was reported for the first time from nature.

U2 - 10.1016/j.chroma.2013.05.032

DO - 10.1016/j.chroma.2013.05.032

M3 - Journal article

VL - 1302

SP - 34

EP - 39

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0301-4770

ER -

ID: 45756515