TY - JOUR

T1 - Systematic review on the current knowledge and use of single-cell RNA sequencing in head and neck cancer

AU - Stampe, Helene

AU - Jakobsen, Kathrine Kronberg

AU - Bendtsen, Simone Kloch

AU - Grønhøj, Christian

AU - von Buchwald, Christian

N1 - Publisher Copyright: © 2021 Scandinavian Societies for Medical Microbiology and Pathology

PY - 2021

Y1 - 2021

N2 - Single-cell RNA sequencing (scRNA-seq) is a novel method enabling genetic characterization of tumor tissue at a single-cell level. This study systematically reviewed the literature on studies using scRNA-seq to characterize head and neck squamous cell carcinomas (HNSCCs). Seven studies were included, of which two studies performed scRNA-seq on 20 patients in total, and five studies used scRNA-seq data in a subsequent clinical study. The former mentioned two studies found intra-tumoral genetic heterogeneity among malignant cells but genetic uniformity among non-malignant cells. The five latter studies used scRNA-seq data in various ways. Three studies identified biomarkers related to predicting whether a patient would benefit from immunotherapeutic treatment. One study characterized genes related to the perineural invasion. One study identified genes to be used in diagnostics. Further studies performing scRNA-seq on HNSCC are required to continue the ongoing development and use of scRNA-seq.

AB - Single-cell RNA sequencing (scRNA-seq) is a novel method enabling genetic characterization of tumor tissue at a single-cell level. This study systematically reviewed the literature on studies using scRNA-seq to characterize head and neck squamous cell carcinomas (HNSCCs). Seven studies were included, of which two studies performed scRNA-seq on 20 patients in total, and five studies used scRNA-seq data in a subsequent clinical study. The former mentioned two studies found intra-tumoral genetic heterogeneity among malignant cells but genetic uniformity among non-malignant cells. The five latter studies used scRNA-seq data in various ways. Three studies identified biomarkers related to predicting whether a patient would benefit from immunotherapeutic treatment. One study characterized genes related to the perineural invasion. One study identified genes to be used in diagnostics. Further studies performing scRNA-seq on HNSCC are required to continue the ongoing development and use of scRNA-seq.

KW - head and neck cancer

KW - head and neck squamous cell carcinoma

KW - HNSCC

KW - scRNA-seq

KW - Single-cell RNA sequencing

U2 - 10.1111/apm.13173

DO - 10.1111/apm.13173

M3 - Review

C2 - 34561896

AN - SCOPUS:85117838737

VL - 129

SP - 619

EP - 625

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 11

ER -