Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase

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Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme's active site yields insights into the mechanism of action of this important class of enzymes.

OriginalsprogEngelsk
Artikelnummer5961
TidsskriftNature Communications
Vol/bind6
Antal sider9
ISSN2041-1723
DOI
StatusUdgivet - 2015

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