Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
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- Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
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Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
Originalsprog | Engelsk |
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Artikelnummer | e0184496 |
Tidsskrift | PLOS ONE |
Vol/bind | 12 |
Udgave nummer | 10 |
Antal sider | 13 |
ISSN | 1932-6203 |
DOI | |
Status | Udgivet - okt. 2017 |
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ID: 186874013