Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin. / Diedrichsen, Ragna Guldsmed; Harloff-Helleberg, Stine; Werner, Ulrich ; Besenius, Melissa; Leberer, Ekkehard ; Kristensen, Mie; Nielsen, Hanne Mørck.

I: Journal of Controlled Release, Bind 338, 2021, s. 8-21.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Diedrichsen, RG, Harloff-Helleberg, S, Werner, U, Besenius, M, Leberer, E, Kristensen, M & Nielsen, HM 2021, 'Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin', Journal of Controlled Release, bind 338, s. 8-21. https://doi.org/10.1016/j.jconrel.2021.07.030

APA

Diedrichsen, R. G., Harloff-Helleberg, S., Werner, U., Besenius, M., Leberer, E., Kristensen, M., & Nielsen, H. M. (2021). Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin. Journal of Controlled Release, 338, 8-21. https://doi.org/10.1016/j.jconrel.2021.07.030

Vancouver

Diedrichsen RG, Harloff-Helleberg S, Werner U, Besenius M, Leberer E, Kristensen M o.a. Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin. Journal of Controlled Release. 2021;338:8-21. https://doi.org/10.1016/j.jconrel.2021.07.030

Author

Diedrichsen, Ragna Guldsmed ; Harloff-Helleberg, Stine ; Werner, Ulrich ; Besenius, Melissa ; Leberer, Ekkehard ; Kristensen, Mie ; Nielsen, Hanne Mørck. / Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin. I: Journal of Controlled Release. 2021 ; Bind 338. s. 8-21.

Bibtex

@article{3fde42f9d1b04275b5ff540f945fd58b,
title = "Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin",
abstract = "Delivery of therapeutic peptides upon oral administration is highly desired and investigations report that the cell-penetrating peptide (CPP) penetratin and its analogues shuffle and penetramax show potential as carriers to enhance insulin delivery. Exploring this, the specific aim of the present study was to understand the impact that their complexation with a lipidated or non-lipidated therapeutic cargo would have on the delivery, to evaluate the effect of differences in membrane interactions in vitro and in vivo, as well as to deduce the mode of action leading to enhanced delivery. Fundamental biophysical aspects were studied by a range of orthogonal methods. Transepithelial permeation of therapeutic peptide was evaluated using the Caco-2 cell culture model supplemented with epithelial integrity measurements, real-time assessment of the carrier peptide effects on cell viability and on mode of action. Pharmacokinetic and pharmacodynamic (PK/PD) parameters were evaluated following intestinal administration to rats and tissue effects were investigated by histology. The biophysical studies revealed complexation of insulin with shuffle and penetramax, but not with penetratin. This corresponded to enhanced transepithelial permeation of insulin, but not of lipidated insulin, when in physical mixture with shuffle or penetramax. The addition of shuffle and penetramax was associated with a lowering of Caco-2 cell monolayer integrity and viability, where the lowering of cell viability was immediate, but reversible. Insulin delivery in rats was enhanced by shuffle and penetramax and accompanied by a 10–20-fold decrease in blood glucose with immediate effect on the intestinal mucosa. In conclusion, shuffle and penetramax, but not penetratin, demonstrated to be potential candidates as carriers for transmucosal delivery of insulin upon oral administration, and their effect depended on association with both cargo and cell membrane. Interestingly, the present study provides novel mechanistic insight that peptide carrier-induced cargo permeation points towards enhancement via the paracellular route in the tight epithelium. This is different from the anticipated belief being that it is the cell-penetrating capability that facilitate transepithelial cargo permeation via a transcellular route.",
author = "Diedrichsen, {Ragna Guldsmed} and Stine Harloff-Helleberg and Ulrich Werner and Melissa Besenius and Ekkehard Leberer and Mie Kristensen and Nielsen, {Hanne M{\o}rck}",
year = "2021",
doi = "10.1016/j.jconrel.2021.07.030",
language = "English",
volume = "338",
pages = "8--21",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Revealing the importance of carrier-cargo association in delivery of insulin and lipidated insulin

AU - Diedrichsen, Ragna Guldsmed

AU - Harloff-Helleberg, Stine

AU - Werner, Ulrich

AU - Besenius, Melissa

AU - Leberer, Ekkehard

AU - Kristensen, Mie

AU - Nielsen, Hanne Mørck

PY - 2021

Y1 - 2021

N2 - Delivery of therapeutic peptides upon oral administration is highly desired and investigations report that the cell-penetrating peptide (CPP) penetratin and its analogues shuffle and penetramax show potential as carriers to enhance insulin delivery. Exploring this, the specific aim of the present study was to understand the impact that their complexation with a lipidated or non-lipidated therapeutic cargo would have on the delivery, to evaluate the effect of differences in membrane interactions in vitro and in vivo, as well as to deduce the mode of action leading to enhanced delivery. Fundamental biophysical aspects were studied by a range of orthogonal methods. Transepithelial permeation of therapeutic peptide was evaluated using the Caco-2 cell culture model supplemented with epithelial integrity measurements, real-time assessment of the carrier peptide effects on cell viability and on mode of action. Pharmacokinetic and pharmacodynamic (PK/PD) parameters were evaluated following intestinal administration to rats and tissue effects were investigated by histology. The biophysical studies revealed complexation of insulin with shuffle and penetramax, but not with penetratin. This corresponded to enhanced transepithelial permeation of insulin, but not of lipidated insulin, when in physical mixture with shuffle or penetramax. The addition of shuffle and penetramax was associated with a lowering of Caco-2 cell monolayer integrity and viability, where the lowering of cell viability was immediate, but reversible. Insulin delivery in rats was enhanced by shuffle and penetramax and accompanied by a 10–20-fold decrease in blood glucose with immediate effect on the intestinal mucosa. In conclusion, shuffle and penetramax, but not penetratin, demonstrated to be potential candidates as carriers for transmucosal delivery of insulin upon oral administration, and their effect depended on association with both cargo and cell membrane. Interestingly, the present study provides novel mechanistic insight that peptide carrier-induced cargo permeation points towards enhancement via the paracellular route in the tight epithelium. This is different from the anticipated belief being that it is the cell-penetrating capability that facilitate transepithelial cargo permeation via a transcellular route.

AB - Delivery of therapeutic peptides upon oral administration is highly desired and investigations report that the cell-penetrating peptide (CPP) penetratin and its analogues shuffle and penetramax show potential as carriers to enhance insulin delivery. Exploring this, the specific aim of the present study was to understand the impact that their complexation with a lipidated or non-lipidated therapeutic cargo would have on the delivery, to evaluate the effect of differences in membrane interactions in vitro and in vivo, as well as to deduce the mode of action leading to enhanced delivery. Fundamental biophysical aspects were studied by a range of orthogonal methods. Transepithelial permeation of therapeutic peptide was evaluated using the Caco-2 cell culture model supplemented with epithelial integrity measurements, real-time assessment of the carrier peptide effects on cell viability and on mode of action. Pharmacokinetic and pharmacodynamic (PK/PD) parameters were evaluated following intestinal administration to rats and tissue effects were investigated by histology. The biophysical studies revealed complexation of insulin with shuffle and penetramax, but not with penetratin. This corresponded to enhanced transepithelial permeation of insulin, but not of lipidated insulin, when in physical mixture with shuffle or penetramax. The addition of shuffle and penetramax was associated with a lowering of Caco-2 cell monolayer integrity and viability, where the lowering of cell viability was immediate, but reversible. Insulin delivery in rats was enhanced by shuffle and penetramax and accompanied by a 10–20-fold decrease in blood glucose with immediate effect on the intestinal mucosa. In conclusion, shuffle and penetramax, but not penetratin, demonstrated to be potential candidates as carriers for transmucosal delivery of insulin upon oral administration, and their effect depended on association with both cargo and cell membrane. Interestingly, the present study provides novel mechanistic insight that peptide carrier-induced cargo permeation points towards enhancement via the paracellular route in the tight epithelium. This is different from the anticipated belief being that it is the cell-penetrating capability that facilitate transepithelial cargo permeation via a transcellular route.

U2 - 10.1016/j.jconrel.2021.07.030

DO - 10.1016/j.jconrel.2021.07.030

M3 - Journal article

C2 - 34298056

VL - 338

SP - 8

EP - 21

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

ER -

ID: 275325548