Rational Design of alpha-helical antimicrobial peptides: DOs and DON’Ts
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Rational Design of alpha-helical antimicrobial peptides: DOs and DON’Ts. / Uggerhøj, Lars Erik; Poulsen, Tanja Juul; Munk, Jens K.; Fredborg, M.; Sondergaard, T. E.; Frimodt-Møller, N.; Hansen, Paul Robert; Wimmer, Reinhard.
I: ChemBioChem, Bind 16, Nr. 2, 2015, s. 242–253.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Rational Design of alpha-helical antimicrobial peptides: DOs and DON’Ts
AU - Uggerhøj, Lars Erik
AU - Poulsen, Tanja Juul
AU - Munk, Jens K.
AU - Fredborg, M.
AU - Sondergaard, T. E.
AU - Frimodt-Møller, N.
AU - Hansen, Paul Robert
AU - Wimmer, Reinhard
PY - 2015
Y1 - 2015
N2 - Antimicrobial peptides (AMPs) are promising candidates for battling multiresistant bacteria. Despite extensive research, structure–activity relationships of AMPs are not fully understood, and there is a lack of structural data relating to AMPs in lipids. Here we present the NMR structure of anoplin (GLLKRIKTLL-NH2) in a micellar environment. A vast library of substitutions was designed and tested for antimicrobial and hemolytic activity, as well as for changes in structure and lipid interactions. This showed that improvement of antimicrobial activity without concomitant introduction of strong hemolytic activity can be achieved through subtle increases in the hydrophobicity of the hydrophobic face or through subtle increases in the polarity of the hydrophilic face of the helix, or—most efficiently—a combination of both. A set of guidelines based on the results is given, for assistance in how to modify cationic α-helical AMPs in order to control activity and selectivity. The guidelines are finally tested on a different peptide.
AB - Antimicrobial peptides (AMPs) are promising candidates for battling multiresistant bacteria. Despite extensive research, structure–activity relationships of AMPs are not fully understood, and there is a lack of structural data relating to AMPs in lipids. Here we present the NMR structure of anoplin (GLLKRIKTLL-NH2) in a micellar environment. A vast library of substitutions was designed and tested for antimicrobial and hemolytic activity, as well as for changes in structure and lipid interactions. This showed that improvement of antimicrobial activity without concomitant introduction of strong hemolytic activity can be achieved through subtle increases in the hydrophobicity of the hydrophobic face or through subtle increases in the polarity of the hydrophilic face of the helix, or—most efficiently—a combination of both. A set of guidelines based on the results is given, for assistance in how to modify cationic α-helical AMPs in order to control activity and selectivity. The guidelines are finally tested on a different peptide.
U2 - 10.1002/cbic.201402581
DO - 10.1002/cbic.201402581
M3 - Journal article
C2 - 25530580
VL - 16
SP - 242
EP - 253
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 2
ER -
ID: 127339740