Rat pancreas secretes particulate ecto-nucleotidase CD39

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Standard

Rat pancreas secretes particulate ecto-nucleotidase CD39. / Sørensen, Christiane Elisabeth; Amstrup, Jan; Rasmussen, Hans N; Ankorina-Stark, Ieva; Novak, Ivana.

I: Journal of Physiology, Bind 551, Nr. 3, 2003, s. 881-892.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Sørensen, CE, Amstrup, J, Rasmussen, HN, Ankorina-Stark, I & Novak, I 2003, 'Rat pancreas secretes particulate ecto-nucleotidase CD39', Journal of Physiology, bind 551, nr. 3, s. 881-892. https://doi.org/10.1113/jphysiol.2003.049411

APA

Sørensen, C. E., Amstrup, J., Rasmussen, H. N., Ankorina-Stark, I., & Novak, I. (2003). Rat pancreas secretes particulate ecto-nucleotidase CD39. Journal of Physiology, 551(3), 881-892. https://doi.org/10.1113/jphysiol.2003.049411

Vancouver

Sørensen CE, Amstrup J, Rasmussen HN, Ankorina-Stark I, Novak I. Rat pancreas secretes particulate ecto-nucleotidase CD39. Journal of Physiology. 2003;551(3):881-892. https://doi.org/10.1113/jphysiol.2003.049411

Author

Sørensen, Christiane Elisabeth ; Amstrup, Jan ; Rasmussen, Hans N ; Ankorina-Stark, Ieva ; Novak, Ivana. / Rat pancreas secretes particulate ecto-nucleotidase CD39. I: Journal of Physiology. 2003 ; Bind 551, Nr. 3. s. 881-892.

Bibtex

@article{1432dce0b18411ddb04f000ea68e967b,
title = "Rat pancreas secretes particulate ecto-nucleotidase CD39",
abstract = "In exocrine pancreas, acini release ATP and the excurrent ducts express several types of purinergic P2 receptors. Thereby, ATP, or its hydrolytic products, might play a role as a paracrine regulator between acini and ducts. The aim of the present study was to elucidate whether this acinar-ductal signalling is regulated by nucleotidase(s), and to characterize and localize one of the nucleotidases within the rat pancreas. Using RT-PCR and Western blotting we show that pancreas expresses the full length ecto-nucleoside triphosphate diphosphohydrolase, CD39. Immunofluorescence shows CD39 localization on basolateral membranes of acini and intracellularly. In small intercalated/ interlobular ducts, CD39 immunofluorescence was localized on the luminal membranes, while in larger ducts it was localized on the basolateral membranes. Upon stimulation with cholecystokinin-octapeptide-8 (CCK-8), acinar CD39 relocalizes in clusters towards the lumen and is secreted. As a result, pancreatic juice collected from intact pancreas stimulated with CCK-8 contained nucleotidase activity, including that of CD39, and no detectable amounts of ATP. Anti-CD39 antibodies detected the full length (78 kDa) CD39 in pancreatic juice. This CD39 was confined only to the particulate and not to the soluble fraction of CCK-8-stimulated secretion. No CD39 activity was detected in secretion stimulated by secretin. The role of secreted particulate, possibly microsomal, CD39 would be to regulate intraluminal ATP concentrations within the ductal tree. In conclusion, we show a novel inducible release of full length particulate CD39, and propose its role in the physiological context of pancreatic secretion.",
author = "S{\o}rensen, {Christiane Elisabeth} and Jan Amstrup and Rasmussen, {Hans N} and Ieva Ankorina-Stark and Ivana Novak",
note = "Keywords: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Blotting, Western; Female; Gene Expression Regulation, Enzymologic; Pancreas; Pancreatic Juice; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction",
year = "2003",
doi = "10.1113/jphysiol.2003.049411",
language = "English",
volume = "551",
pages = "881--892",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Rat pancreas secretes particulate ecto-nucleotidase CD39

AU - Sørensen, Christiane Elisabeth

AU - Amstrup, Jan

AU - Rasmussen, Hans N

AU - Ankorina-Stark, Ieva

AU - Novak, Ivana

N1 - Keywords: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Blotting, Western; Female; Gene Expression Regulation, Enzymologic; Pancreas; Pancreatic Juice; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction

PY - 2003

Y1 - 2003

N2 - In exocrine pancreas, acini release ATP and the excurrent ducts express several types of purinergic P2 receptors. Thereby, ATP, or its hydrolytic products, might play a role as a paracrine regulator between acini and ducts. The aim of the present study was to elucidate whether this acinar-ductal signalling is regulated by nucleotidase(s), and to characterize and localize one of the nucleotidases within the rat pancreas. Using RT-PCR and Western blotting we show that pancreas expresses the full length ecto-nucleoside triphosphate diphosphohydrolase, CD39. Immunofluorescence shows CD39 localization on basolateral membranes of acini and intracellularly. In small intercalated/ interlobular ducts, CD39 immunofluorescence was localized on the luminal membranes, while in larger ducts it was localized on the basolateral membranes. Upon stimulation with cholecystokinin-octapeptide-8 (CCK-8), acinar CD39 relocalizes in clusters towards the lumen and is secreted. As a result, pancreatic juice collected from intact pancreas stimulated with CCK-8 contained nucleotidase activity, including that of CD39, and no detectable amounts of ATP. Anti-CD39 antibodies detected the full length (78 kDa) CD39 in pancreatic juice. This CD39 was confined only to the particulate and not to the soluble fraction of CCK-8-stimulated secretion. No CD39 activity was detected in secretion stimulated by secretin. The role of secreted particulate, possibly microsomal, CD39 would be to regulate intraluminal ATP concentrations within the ductal tree. In conclusion, we show a novel inducible release of full length particulate CD39, and propose its role in the physiological context of pancreatic secretion.

AB - In exocrine pancreas, acini release ATP and the excurrent ducts express several types of purinergic P2 receptors. Thereby, ATP, or its hydrolytic products, might play a role as a paracrine regulator between acini and ducts. The aim of the present study was to elucidate whether this acinar-ductal signalling is regulated by nucleotidase(s), and to characterize and localize one of the nucleotidases within the rat pancreas. Using RT-PCR and Western blotting we show that pancreas expresses the full length ecto-nucleoside triphosphate diphosphohydrolase, CD39. Immunofluorescence shows CD39 localization on basolateral membranes of acini and intracellularly. In small intercalated/ interlobular ducts, CD39 immunofluorescence was localized on the luminal membranes, while in larger ducts it was localized on the basolateral membranes. Upon stimulation with cholecystokinin-octapeptide-8 (CCK-8), acinar CD39 relocalizes in clusters towards the lumen and is secreted. As a result, pancreatic juice collected from intact pancreas stimulated with CCK-8 contained nucleotidase activity, including that of CD39, and no detectable amounts of ATP. Anti-CD39 antibodies detected the full length (78 kDa) CD39 in pancreatic juice. This CD39 was confined only to the particulate and not to the soluble fraction of CCK-8-stimulated secretion. No CD39 activity was detected in secretion stimulated by secretin. The role of secreted particulate, possibly microsomal, CD39 would be to regulate intraluminal ATP concentrations within the ductal tree. In conclusion, we show a novel inducible release of full length particulate CD39, and propose its role in the physiological context of pancreatic secretion.

U2 - 10.1113/jphysiol.2003.049411

DO - 10.1113/jphysiol.2003.049411

M3 - Journal article

C2 - 12832497

VL - 551

SP - 881

EP - 892

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 3

ER -

ID: 8569815