Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue

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Standard

Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue. / Blomster, Linda V; Strøbaek, Dorte; Hougaard, Charlotte; Klein, Jessica; Pinborg, Lars H; Mikkelsen, Jens D; Christophersen, Palle.

I: Glia, Bind 64, Nr. 12, 12.2016, s. 2065-2078.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Blomster, LV, Strøbaek, D, Hougaard, C, Klein, J, Pinborg, LH, Mikkelsen, JD & Christophersen, P 2016, 'Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue', Glia, bind 64, nr. 12, s. 2065-2078. https://doi.org/10.1002/glia.23040

APA

Blomster, L. V., Strøbaek, D., Hougaard, C., Klein, J., Pinborg, L. H., Mikkelsen, J. D., & Christophersen, P. (2016). Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue. Glia, 64(12), 2065-2078. https://doi.org/10.1002/glia.23040

Vancouver

Blomster LV, Strøbaek D, Hougaard C, Klein J, Pinborg LH, Mikkelsen JD o.a. Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue. Glia. 2016 dec;64(12):2065-2078. https://doi.org/10.1002/glia.23040

Author

Blomster, Linda V ; Strøbaek, Dorte ; Hougaard, Charlotte ; Klein, Jessica ; Pinborg, Lars H ; Mikkelsen, Jens D ; Christophersen, Palle. / Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue. I: Glia. 2016 ; Bind 64, Nr. 12. s. 2065-2078.

Bibtex

@article{c4b89c4ee1064334a911bed7bd3aac99,
title = "Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue",
abstract = "The KCa 3.1 channel (KCNN4) is an important modulator of microglia responses in rodents, but no information exists on functional expression on microglia from human adults. We isolated and cultured microglia (max 1{\%} astrocytes, no neurons or oligodendrocytes) from neocortex surgically removed from epilepsy patients and employed electrophysiological whole-cell measurements and selective pharmacological tools to elucidate functional expression of KCa 3.1. The channel expression was demonstrated as a significant increase in the voltage-independent current by NS309, a KCa 3.1/KCa 2 activator, followed by full inhibition upon co-application with NS6180, a highly selective KCa 3.1 inhibitor. A major fraction (79{\%}) of unstimulated human microglia expressed KCa 3.1, and the difference in current between full activation and inhibition (ΔKCa 3.1) was estimated at 292 ± 48 pA at -40 mV (n = 75), which equals at least 585 channels per cell. Serial KCa 3.1 activation/inhibition significantly hyperpolarized/depolarized the membrane potential. The isolated human microglia were potently activated by lipopolysaccharide (LPS) shown as a prominent increase in TNF-α production. However, incubation with LPS neither changed the KCa 3.1 current nor the fraction of KCa 3.1 expressing cells. In contrast, the anti-inflammatory cytokine IL-4 slightly increased the KCa 3.1 current per cell, but as the membrane area also increased, there was no significant change in channel density. A large fraction of the microglia also expressed a voltage-dependent current sensitive to the KCa 1.1 modulators NS1619 and Paxilline and an inward-rectifying current with the characteristics of a Kir channel. The high functional expression of KCa 3.1 in microglia from epilepsy patients accentuates the need for further investigations of its role in neuropathological processes. GLIA 2016;64:2065-2078.",
keywords = "Journal Article",
author = "Blomster, {Linda V} and Dorte Str{\o}baek and Charlotte Hougaard and Jessica Klein and Pinborg, {Lars H} and Mikkelsen, {Jens D} and Palle. Christophersen",
note = "{\circledC} 2016 Wiley Periodicals, Inc.",
year = "2016",
month = "12",
doi = "10.1002/glia.23040",
language = "English",
volume = "64",
pages = "2065--2078",
journal = "Glia",
issn = "0894-1491",
publisher = "JohnWiley & Sons, Inc.",
number = "12",

}

RIS

TY - JOUR

T1 - Quantification of the functional expression of the Ca2+‐activated K+ channel KCa3.1 on microglia from adult human neocortical tissue

AU - Blomster, Linda V

AU - Strøbaek, Dorte

AU - Hougaard, Charlotte

AU - Klein, Jessica

AU - Pinborg, Lars H

AU - Mikkelsen, Jens D

AU - Christophersen, Palle.

N1 - © 2016 Wiley Periodicals, Inc.

PY - 2016/12

Y1 - 2016/12

N2 - The KCa 3.1 channel (KCNN4) is an important modulator of microglia responses in rodents, but no information exists on functional expression on microglia from human adults. We isolated and cultured microglia (max 1% astrocytes, no neurons or oligodendrocytes) from neocortex surgically removed from epilepsy patients and employed electrophysiological whole-cell measurements and selective pharmacological tools to elucidate functional expression of KCa 3.1. The channel expression was demonstrated as a significant increase in the voltage-independent current by NS309, a KCa 3.1/KCa 2 activator, followed by full inhibition upon co-application with NS6180, a highly selective KCa 3.1 inhibitor. A major fraction (79%) of unstimulated human microglia expressed KCa 3.1, and the difference in current between full activation and inhibition (ΔKCa 3.1) was estimated at 292 ± 48 pA at -40 mV (n = 75), which equals at least 585 channels per cell. Serial KCa 3.1 activation/inhibition significantly hyperpolarized/depolarized the membrane potential. The isolated human microglia were potently activated by lipopolysaccharide (LPS) shown as a prominent increase in TNF-α production. However, incubation with LPS neither changed the KCa 3.1 current nor the fraction of KCa 3.1 expressing cells. In contrast, the anti-inflammatory cytokine IL-4 slightly increased the KCa 3.1 current per cell, but as the membrane area also increased, there was no significant change in channel density. A large fraction of the microglia also expressed a voltage-dependent current sensitive to the KCa 1.1 modulators NS1619 and Paxilline and an inward-rectifying current with the characteristics of a Kir channel. The high functional expression of KCa 3.1 in microglia from epilepsy patients accentuates the need for further investigations of its role in neuropathological processes. GLIA 2016;64:2065-2078.

AB - The KCa 3.1 channel (KCNN4) is an important modulator of microglia responses in rodents, but no information exists on functional expression on microglia from human adults. We isolated and cultured microglia (max 1% astrocytes, no neurons or oligodendrocytes) from neocortex surgically removed from epilepsy patients and employed electrophysiological whole-cell measurements and selective pharmacological tools to elucidate functional expression of KCa 3.1. The channel expression was demonstrated as a significant increase in the voltage-independent current by NS309, a KCa 3.1/KCa 2 activator, followed by full inhibition upon co-application with NS6180, a highly selective KCa 3.1 inhibitor. A major fraction (79%) of unstimulated human microglia expressed KCa 3.1, and the difference in current between full activation and inhibition (ΔKCa 3.1) was estimated at 292 ± 48 pA at -40 mV (n = 75), which equals at least 585 channels per cell. Serial KCa 3.1 activation/inhibition significantly hyperpolarized/depolarized the membrane potential. The isolated human microglia were potently activated by lipopolysaccharide (LPS) shown as a prominent increase in TNF-α production. However, incubation with LPS neither changed the KCa 3.1 current nor the fraction of KCa 3.1 expressing cells. In contrast, the anti-inflammatory cytokine IL-4 slightly increased the KCa 3.1 current per cell, but as the membrane area also increased, there was no significant change in channel density. A large fraction of the microglia also expressed a voltage-dependent current sensitive to the KCa 1.1 modulators NS1619 and Paxilline and an inward-rectifying current with the characteristics of a Kir channel. The high functional expression of KCa 3.1 in microglia from epilepsy patients accentuates the need for further investigations of its role in neuropathological processes. GLIA 2016;64:2065-2078.

KW - Journal Article

U2 - 10.1002/glia.23040

DO - 10.1002/glia.23040

M3 - Journal article

C2 - 27470924

VL - 64

SP - 2065

EP - 2078

JO - Glia

JF - Glia

SN - 0894-1491

IS - 12

ER -

ID: 180370640