Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection
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Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection. / Møller, Laura Hyrup; Macherius, André; Hansen, Thomas Hesselhøj; Nielsen, Hanne Mørck; Cornett, Claus; Østergaard, Jesper; Stürup, Stefan; Gammelgaard, Bente.
I: Journal of Analytical Atomic Spectrometry, Bind 31, 2016, s. 1877-1884.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection
AU - Møller, Laura Hyrup
AU - Macherius, André
AU - Hansen, Thomas Hesselhøj
AU - Nielsen, Hanne Mørck
AU - Cornett, Claus
AU - Østergaard, Jesper
AU - Stürup, Stefan
AU - Gammelgaard, Bente
PY - 2016
Y1 - 2016
N2 - A method for quantification of a pharmaceutical peptide in human plasma was developed using gradient elution LC-ICP-MS. A membrane desolvation (MD) system was applied to remove organic solvents from the eluent prior to the detection as SO+ in the dynamic reaction cell (DRC) of the ICP-DRC-MS instrument and subsequent quantification by post-column isotope dilution (IDA). Plasma proteins were precipitated prior to analysis. Analytical figures of merit including linearity, precision, LOD, LOQ and accuracy were considered satisfactory for analysis of plasma samples. The selectivity of the developed method was demonstrated for five pharmaceutically relevant peptides: desmopressin, penetratin, substance P, PTH (1-34) and insulin. Preliminary experiments on an ICP-MS/MS system using oxygen to reduce the effect of organic solvents were also performed to compare sensitivity. The results of the study demonstrated that LC-ICP-MS post-column IDA may constitute a valuable additional tool in quantification of non-labelled peptides in the early drug development offering absolute quantification without need of species specific standards.
AB - A method for quantification of a pharmaceutical peptide in human plasma was developed using gradient elution LC-ICP-MS. A membrane desolvation (MD) system was applied to remove organic solvents from the eluent prior to the detection as SO+ in the dynamic reaction cell (DRC) of the ICP-DRC-MS instrument and subsequent quantification by post-column isotope dilution (IDA). Plasma proteins were precipitated prior to analysis. Analytical figures of merit including linearity, precision, LOD, LOQ and accuracy were considered satisfactory for analysis of plasma samples. The selectivity of the developed method was demonstrated for five pharmaceutically relevant peptides: desmopressin, penetratin, substance P, PTH (1-34) and insulin. Preliminary experiments on an ICP-MS/MS system using oxygen to reduce the effect of organic solvents were also performed to compare sensitivity. The results of the study demonstrated that LC-ICP-MS post-column IDA may constitute a valuable additional tool in quantification of non-labelled peptides in the early drug development offering absolute quantification without need of species specific standards.
U2 - 10.1039/C6JA00132G
DO - 10.1039/C6JA00132G
M3 - Journal article
VL - 31
SP - 1877
EP - 1884
JO - Journal of Analytical Atomic Spectrometry
JF - Journal of Analytical Atomic Spectrometry
SN - 0267-9477
ER -
ID: 164887608