Propionic acid secreted from propionibacteria induces NKG2D ligand expression on human-activated T lymphocytes and cancer cells
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Propionic acid secreted from propionibacteria induces NKG2D ligand expression on human-activated T lymphocytes and cancer cells. / Andresen, Lars; Hansen, Karen Aagaard; Jensen, Helle; Pedersen, Stine Helene Falsig; Stougaard, Peter; Hansen, Helle Rüsz; Jurlander, Jesper; Skov, Søren.
I: Journal of Immunology, Bind 183, Nr. 2, 2009, s. 897-906.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Propionic acid secreted from propionibacteria induces NKG2D ligand expression on human-activated T lymphocytes and cancer cells
AU - Andresen, Lars
AU - Hansen, Karen Aagaard
AU - Jensen, Helle
AU - Pedersen, Stine Helene Falsig
AU - Stougaard, Peter
AU - Hansen, Helle Rüsz
AU - Jurlander, Jesper
AU - Skov, Søren
N1 - Keywords: Bacteria; Calcium; Cell Line, Tumor; Hematologic Neoplasms; Histocompatibility Antigens Class I; Humans; Jurkat Cells; Ligands; Lymphocyte Activation; NK Cell Lectin-Like Receptor Subfamily K; Promoter Regions, Genetic; Propionic Acids; T-Lymphocytes; Transcriptional Activation
PY - 2009
Y1 - 2009
N2 - We found that propionic acid secreted from propionibacteria induces expression of the NKG2D ligands MICA/B on activated T lymphocytes and different cancer cells, without affecting MICA/B expression on resting peripheral blood cells. Growth supernatant from propionibacteria or propionate alone could directly stimulate functional MICA/B surface expression and MICA promoter activity by a mechanism dependent on intracellular calcium. Deletion and point mutations further demonstrated that a GC-box motif around -110 from the MICA transcription start site is essential for propionate-mediated MICA promoter activity. Other short-chain fatty acids such as lactate, acetate, and butyrate could also induce MICA/B expression. We observed a striking difference in the molecular signaling pathways that regulate MICA/B. A functional glycolytic pathway was essential for MICA/B expression after exposure to propionate and CMV. In contrast, compounds with histone deacetylase-inhibitory activity such as butyrate and FR901228 stimulated MICA/B expression through a pathway that was not affected by inhibition of glycolysis, clearly suggesting that MICA/B is regulated through different molecular mechanisms. We propose that propionate, produced either by bacteria or during cellular metabolism, has significant immunoregulatory function and may be cancer prophylactic.
AB - We found that propionic acid secreted from propionibacteria induces expression of the NKG2D ligands MICA/B on activated T lymphocytes and different cancer cells, without affecting MICA/B expression on resting peripheral blood cells. Growth supernatant from propionibacteria or propionate alone could directly stimulate functional MICA/B surface expression and MICA promoter activity by a mechanism dependent on intracellular calcium. Deletion and point mutations further demonstrated that a GC-box motif around -110 from the MICA transcription start site is essential for propionate-mediated MICA promoter activity. Other short-chain fatty acids such as lactate, acetate, and butyrate could also induce MICA/B expression. We observed a striking difference in the molecular signaling pathways that regulate MICA/B. A functional glycolytic pathway was essential for MICA/B expression after exposure to propionate and CMV. In contrast, compounds with histone deacetylase-inhibitory activity such as butyrate and FR901228 stimulated MICA/B expression through a pathway that was not affected by inhibition of glycolysis, clearly suggesting that MICA/B is regulated through different molecular mechanisms. We propose that propionate, produced either by bacteria or during cellular metabolism, has significant immunoregulatory function and may be cancer prophylactic.
KW - Bacteria
KW - Calcium
KW - Cell Line, Tumor
KW - Hematologic Neoplasms
KW - Histocompatibility Antigens Class I
KW - Humans
KW - Jurkat Cells
KW - Ligands
KW - Lymphocyte Activation
KW - NK Cell Lectin-Like Receptor Subfamily K
KW - Promoter Regions, Genetic
KW - Propionic Acids
KW - T-Lymphocytes
KW - Transcriptional Activation
U2 - 10.4049/jimmunol.0803014
DO - 10.4049/jimmunol.0803014
M3 - Journal article
C2 - 19553547
VL - 183
SP - 897
EP - 906
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -
ID: 17654243