Probing the Role of Sigma π Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-β-Xylanase

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  • Raushan Kumar Singh
  • Manish Kumar Tiwari
  • In-Won Kim
  • Zhilei Chen
  • Jung-Kul Lee
Chaetomium globosum endo-1,4-β-xylanase (XylCg) is distinguished from other xylanases by its high turnover rate (1,860 s−1), the highest ever reported for fungal xylanases. One conserved amino acid, W48, in the substrate binding pocket of wild-type XylCg was identified as an important residue affecting XylCg's catalytic efficiency.
TidsskriftApplied and Environmental Microbiology
StatusUdgivet - 2012

ID: 229903076