Biochemical methane potential (BMP) assays are widely used to assess feedsocks in oder to control the process of biogas production. However, the continuous evaluation of feedstocks using a BMP assay is expensive, time-consuming and challenging. In this study, Fourier transform mid-infrared photoacoustic spectroscopy (FTIR-PAS) was used to predict the BMP values of 87 urban organic waste (UOW) samples derived from different sources in Denmark. The developed model of BMP prediction showed a coefficient of determination (R²) of 0.86 and a root mean square error (RMSE) of 59.3 mL CH₄/g VS in prediction. The interpretation of the regression coefficients used in the calibration showed a positive correlation with BMP for relatively easily degradable compounds, such as aliphatics, most likely lipids and amides most likely in proteins, while a negative correlation was found with lignin and hemicellulose.