PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional program
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
PR (PRD1-BF1-RIZ1 homologous) domain-containing 16 (PRDM16) drives a brown fat differentiation program, but the mechanisms by which PRDM16 activates brown fat-selective genes have been unclear. Through chromatin immunoprecipitation (ChIP) followed by deep sequencing (ChIP-seq) analyses in brown adipose tissue (BAT), we reveal that PRDM16 binding is highly enriched at a broad set of brown fat-selective genes. Importantly, we found that PRDM16 physically binds to MED1, a component of the Mediator complex, and recruits it to superenhancers at brown fat-selective genes. PRDM16 deficiency in BAT reduces MED1 binding at PRDM16 target sites and causes a fundamental change in chromatin architecture at key brown fat-selective genes. Together, these data indicate that PRDM16 controls chromatin architecture and superenhancer activity in BAT.
Originalsprog | Engelsk |
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Tidsskrift | Genes & Development |
Vol/bind | 29 |
Udgave nummer | 3 |
Sider (fra-til) | 298-307 |
Antal sider | 10 |
ISSN | 0890-9369 |
DOI | |
Status | Udgivet - 1 feb. 2015 |
Eksternt udgivet | Ja |
ID: 199331220