Plasma Proteome Profiling to Assess Human Health and Disease

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Plasma Proteome Profiling to Assess Human Health and Disease. / Geyer, Philipp E; Kulak, Nils A; Pichler, Garwin; Holdt, Lesca M; Teupser, Daniel; Mann, Matthias.

I: Cell Systems, Bind 2, Nr. 3, 23.03.2016, s. 185-95.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Geyer, PE, Kulak, NA, Pichler, G, Holdt, LM, Teupser, D & Mann, M 2016, 'Plasma Proteome Profiling to Assess Human Health and Disease', Cell Systems, bind 2, nr. 3, s. 185-95. https://doi.org/10.1016/j.cels.2016.02.015

APA

Geyer, P. E., Kulak, N. A., Pichler, G., Holdt, L. M., Teupser, D., & Mann, M. (2016). Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems, 2(3), 185-95. https://doi.org/10.1016/j.cels.2016.02.015

Vancouver

Geyer PE, Kulak NA, Pichler G, Holdt LM, Teupser D, Mann M. Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems. 2016 mar. 23;2(3):185-95. https://doi.org/10.1016/j.cels.2016.02.015

Author

Geyer, Philipp E ; Kulak, Nils A ; Pichler, Garwin ; Holdt, Lesca M ; Teupser, Daniel ; Mann, Matthias. / Plasma Proteome Profiling to Assess Human Health and Disease. I: Cell Systems. 2016 ; Bind 2, Nr. 3. s. 185-95.

Bibtex

@article{6049896d4611461794363984940bac3e,
title = "Plasma Proteome Profiling to Assess Human Health and Disease",
abstract = "Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust {"}plasma proteome profiling{"} pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.",
keywords = "Journal Article",
author = "Geyer, {Philipp E} and Kulak, {Nils A} and Garwin Pichler and Holdt, {Lesca M} and Daniel Teupser and Matthias Mann",
note = "Copyright {\textcopyright} 2016 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2016",
month = mar,
day = "23",
doi = "10.1016/j.cels.2016.02.015",
language = "English",
volume = "2",
pages = "185--95",
journal = "Cell Systems",
issn = "2405-4712",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Plasma Proteome Profiling to Assess Human Health and Disease

AU - Geyer, Philipp E

AU - Kulak, Nils A

AU - Pichler, Garwin

AU - Holdt, Lesca M

AU - Teupser, Daniel

AU - Mann, Matthias

N1 - Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2016/3/23

Y1 - 2016/3/23

N2 - Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.

AB - Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.

KW - Journal Article

U2 - 10.1016/j.cels.2016.02.015

DO - 10.1016/j.cels.2016.02.015

M3 - Journal article

C2 - 27135364

VL - 2

SP - 185

EP - 195

JO - Cell Systems

JF - Cell Systems

SN - 2405-4712

IS - 3

ER -

ID: 165939283