Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes

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Standard

Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes. / Gawronski, Piotr; Jensen, Poul Erik; Karpinski, Stanislaw; Leister, Dario Michael; Scharff, Lars.

I: Plant Physiology, Bind 176, Nr. 3, 2018, s. 2557-2569.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Gawronski, P, Jensen, PE, Karpinski, S, Leister, DM & Scharff, L 2018, 'Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes', Plant Physiology, bind 176, nr. 3, s. 2557-2569. https://doi.org/10.1104/pp.17.01564

APA

Gawronski, P., Jensen, P. E., Karpinski, S., Leister, D. M., & Scharff, L. (2018). Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes. Plant Physiology, 176(3), 2557-2569. https://doi.org/10.1104/pp.17.01564

Vancouver

Gawronski P, Jensen PE, Karpinski S, Leister DM, Scharff L. Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes. Plant Physiology. 2018;176(3):2557-2569. https://doi.org/10.1104/pp.17.01564

Author

Gawronski, Piotr ; Jensen, Poul Erik ; Karpinski, Stanislaw ; Leister, Dario Michael ; Scharff, Lars. / Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes. I: Plant Physiology. 2018 ; Bind 176, Nr. 3. s. 2557-2569.

Bibtex

@article{00a94625d0a245a5bf0855ea77899b6c,
title = "Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes",
abstract = "Many mRNAs contain pause sites that briefly interrupt the progress of translation. Specific features that induce ribosome pausing have been described; however, their individual contributions to pause-site formation, and the overall biological significance of ribosome pausing, remain largely unclear. We have taken advantage of the compact genome of chloroplasts to carry out a plastid genome-wide survey of pause sites, as a basis for studying the impact of pausing on post-translational processes. Based on ribosomal profiling of Arabidopsis thaliana chloroplast mRNAs, we demonstrate that a combination of factors - mRNA secondary structure, internal Shine-Dalgarno sequences, and positively charged amino acids in the nascent peptide chain - explains 95% of the major pause sites on plastid mRNAs, while codon usage has little impact. The distribution of the pause sites is non-random and conforms to distinct patterns in the vicinity of sequences coding for transmembrane domains - which depend on their orientation within the membrane - as well as being next to sequences coding for cofactor binding sites. We found strong indications that the mechanisms causing ribosomal pausing and at least some of the ribosomes pause sites are conserved between distantly related plant species. In addition, the positions of features that cause pausing are well conserved in photoautotrophic plants, but less so in their non-photosynthetic, parasitic relatives, implying that the synthesis and assembly of photosynthetic multiprotein complexes requires localized ribosome pausing.",
keywords = "Journal Article",
author = "Piotr Gawronski and Jensen, {Poul Erik} and Stanislaw Karpinski and Leister, {Dario Michael} and Lars Scharff",
note = "{copyright, serif} 2018 American Society of Plant Biologists. All rights reserved.",
year = "2018",
doi = "10.1104/pp.17.01564",
language = "English",
volume = "176",
pages = "2557--2569",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

RIS

TY - JOUR

T1 - Pausing of chloroplast ribosomes is induced by multiple features and is linked to the assembly of photosynthetic complexes

AU - Gawronski, Piotr

AU - Jensen, Poul Erik

AU - Karpinski, Stanislaw

AU - Leister, Dario Michael

AU - Scharff, Lars

N1 - {copyright, serif} 2018 American Society of Plant Biologists. All rights reserved.

PY - 2018

Y1 - 2018

N2 - Many mRNAs contain pause sites that briefly interrupt the progress of translation. Specific features that induce ribosome pausing have been described; however, their individual contributions to pause-site formation, and the overall biological significance of ribosome pausing, remain largely unclear. We have taken advantage of the compact genome of chloroplasts to carry out a plastid genome-wide survey of pause sites, as a basis for studying the impact of pausing on post-translational processes. Based on ribosomal profiling of Arabidopsis thaliana chloroplast mRNAs, we demonstrate that a combination of factors - mRNA secondary structure, internal Shine-Dalgarno sequences, and positively charged amino acids in the nascent peptide chain - explains 95% of the major pause sites on plastid mRNAs, while codon usage has little impact. The distribution of the pause sites is non-random and conforms to distinct patterns in the vicinity of sequences coding for transmembrane domains - which depend on their orientation within the membrane - as well as being next to sequences coding for cofactor binding sites. We found strong indications that the mechanisms causing ribosomal pausing and at least some of the ribosomes pause sites are conserved between distantly related plant species. In addition, the positions of features that cause pausing are well conserved in photoautotrophic plants, but less so in their non-photosynthetic, parasitic relatives, implying that the synthesis and assembly of photosynthetic multiprotein complexes requires localized ribosome pausing.

AB - Many mRNAs contain pause sites that briefly interrupt the progress of translation. Specific features that induce ribosome pausing have been described; however, their individual contributions to pause-site formation, and the overall biological significance of ribosome pausing, remain largely unclear. We have taken advantage of the compact genome of chloroplasts to carry out a plastid genome-wide survey of pause sites, as a basis for studying the impact of pausing on post-translational processes. Based on ribosomal profiling of Arabidopsis thaliana chloroplast mRNAs, we demonstrate that a combination of factors - mRNA secondary structure, internal Shine-Dalgarno sequences, and positively charged amino acids in the nascent peptide chain - explains 95% of the major pause sites on plastid mRNAs, while codon usage has little impact. The distribution of the pause sites is non-random and conforms to distinct patterns in the vicinity of sequences coding for transmembrane domains - which depend on their orientation within the membrane - as well as being next to sequences coding for cofactor binding sites. We found strong indications that the mechanisms causing ribosomal pausing and at least some of the ribosomes pause sites are conserved between distantly related plant species. In addition, the positions of features that cause pausing are well conserved in photoautotrophic plants, but less so in their non-photosynthetic, parasitic relatives, implying that the synthesis and assembly of photosynthetic multiprotein complexes requires localized ribosome pausing.

KW - Journal Article

U2 - 10.1104/pp.17.01564

DO - 10.1104/pp.17.01564

M3 - Journal article

C2 - 29298822

VL - 176

SP - 2557

EP - 2569

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -

ID: 188111588