TY - JOUR

T1 - Nano-nutrition of chicken embryos. The effect of silver nanoparticles and glutamine on molecular responses, and the morphology of pectoral muscle

T2 - the effect of silver nanoparticles and glutamine on molecular responses, and the morphology of pectoral muscle

AU - Sawosz, Filip

AU - Pineda, Lane Manalili

AU - Hotowy, Anna Malgorzata

AU - Hyttel, Poul

AU - Sawosz, Ewa

AU - Szmidt, Maciek

AU - Niemiec, Tomasz

AU - Chwalibog, André

PY - 2012

Y1 - 2012

N2 - Background: It has been demonstrated that concentrations of certain amino acids in the egg, in late-term embryos, are not sufficient to fully support embryonic development. One of the methods to assure an adequate nutrient content in the egg is in ovo administration of nutrients, which increases hatching weight and the size of the breast muscle. The small size of silver nanoparticles allows for penetration inside tissues and even enables them to cross cell membranes. Indeed it has been shown that nanoparticles of silver applied in ovo can up-regulate the expression of fibroblast growth factor and vascular endothelial growth factor. We have therefore tested if silver nanoparticles can affect muscle development of chicken embryos and, furthermore, if they can be used in in ovo nutrition as carriers of nutrients e.g. glutamine into muscle cells. Methods: 160 broiler eggs were randomly divided into the control group (Control) without injection and injected groups with hydrocolloids of nanoparticles of silver (Nano-Ag), glutamine (Glu) and the complex of nanoparticles of silver and glutamine (Nano-Ag/Glu). The embryos were evaluated on day 20 of incubation. Samples of the breast muscles were collected for gene expression analysis or fixed for electron microscopy preparation. Results: Results indicate a significant role of silver nanoparticles and the complex Nano-Ag/Glu in muscle development. Conclusion: Nanoparticles of Ag, glutamine and the complexes of Ag and glutamine were without negative effects on embryo development. Nanoparticles of Ag and the complex of Ag with glutamine increased the number of nuclei per cell number and also fiber area. Furthermore, the complex of Ag with glutamine increased muscle mass.

AB - Background: It has been demonstrated that concentrations of certain amino acids in the egg, in late-term embryos, are not sufficient to fully support embryonic development. One of the methods to assure an adequate nutrient content in the egg is in ovo administration of nutrients, which increases hatching weight and the size of the breast muscle. The small size of silver nanoparticles allows for penetration inside tissues and even enables them to cross cell membranes. Indeed it has been shown that nanoparticles of silver applied in ovo can up-regulate the expression of fibroblast growth factor and vascular endothelial growth factor. We have therefore tested if silver nanoparticles can affect muscle development of chicken embryos and, furthermore, if they can be used in in ovo nutrition as carriers of nutrients e.g. glutamine into muscle cells. Methods: 160 broiler eggs were randomly divided into the control group (Control) without injection and injected groups with hydrocolloids of nanoparticles of silver (Nano-Ag), glutamine (Glu) and the complex of nanoparticles of silver and glutamine (Nano-Ag/Glu). The embryos were evaluated on day 20 of incubation. Samples of the breast muscles were collected for gene expression analysis or fixed for electron microscopy preparation. Results: Results indicate a significant role of silver nanoparticles and the complex Nano-Ag/Glu in muscle development. Conclusion: Nanoparticles of Ag, glutamine and the complexes of Ag and glutamine were without negative effects on embryo development. Nanoparticles of Ag and the complex of Ag with glutamine increased the number of nuclei per cell number and also fiber area. Furthermore, the complex of Ag with glutamine increased muscle mass.

KW - Former LIFE faculty

KW - silver nanoparticles

KW - muscle

KW - glutamine

KW - nutrition

KW - gene expression

KW - chicken embryo

U2 - 10.7136/bjccsb.2012.2.0029

DO - 10.7136/bjccsb.2012.2.0029

M3 - Journal article

VL - 2

SP - 29

EP - 45

JO - Baltic Journal of Comparative & Clinical Systems Biology

JF - Baltic Journal of Comparative & Clinical Systems Biology

SN - 2245-2826

ER -