Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise
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Muscle interstitial potassium kinetics during intense exhaustive exercise : effect of previous arm exercise. / Nordsborg, Nikolai; Mohr, Magni; Pedersen, Lasse Dannemann; Nielsen, Jens Jung; Langberg, Henning; Bangsbo, Jens.
I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 285, Nr. 1, 2003, s. R143-R148.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Muscle interstitial potassium kinetics during intense exhaustive exercise
T2 - effect of previous arm exercise
AU - Nordsborg, Nikolai
AU - Mohr, Magni
AU - Pedersen, Lasse Dannemann
AU - Nielsen, Jens Jung
AU - Langberg, Henning
AU - Bangsbo, Jens
N1 - PUF 2003 5200 063
PY - 2003
Y1 - 2003
N2 - Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced, when exhaustive leg exercise was performed on two different days, with a between-day difference of approximately 0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7 +/- 0.3 vs. 4.0 +/- 0.3 min; P <0.05). Furthermore, [K+]i was higher from 0 to 1.5 min of the intense leg exercise period in AL compared with L (9.2 +/- 0.7 vs. 6.4 +/- 0.9 mM; P <0.001) and at exhaustion (11.9 +/- 0.5 vs. 10.3 +/- 0.6 mM; P <0.05). The dialysate content of carnosine was elevated by exercise, but low-intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low-intensity exercise is performed before the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.
AB - Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced, when exhaustive leg exercise was performed on two different days, with a between-day difference of approximately 0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7 +/- 0.3 vs. 4.0 +/- 0.3 min; P <0.05). Furthermore, [K+]i was higher from 0 to 1.5 min of the intense leg exercise period in AL compared with L (9.2 +/- 0.7 vs. 6.4 +/- 0.9 mM; P <0.001) and at exhaustion (11.9 +/- 0.5 vs. 10.3 +/- 0.6 mM; P <0.05). The dialysate content of carnosine was elevated by exercise, but low-intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low-intensity exercise is performed before the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.
KW - Acidosis
KW - Adult
KW - Arm
KW - Carnosine
KW - Extracellular Space
KW - Humans
KW - Hydrogen-Ion Concentration
KW - Kinetics
KW - Leg
KW - Male
KW - Microdialysis
KW - Muscle Fatigue
KW - Muscle, Skeletal
KW - Physical Endurance
KW - Potassium
U2 - 10.1152/ajpregu.00029.2003
DO - 10.1152/ajpregu.00029.2003
M3 - Journal article
C2 - 12663256
VL - 285
SP - R143-R148
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 1
ER -
ID: 124220