TY - JOUR

T1 - Minimal information for studies of extracellular vesicles 2018 (MISEV2018)

T2 - a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

AU - Théry, Clotilde

AU - Witwer, Kenneth W

AU - Aikawa, Elena

AU - Alcaraz, Maria Jose

AU - Anderson, Johnathon D

AU - Andriantsitohaina, Ramaroson

AU - Antoniou, Anna

AU - Arab, Tanina

AU - Archer, Fabienne

AU - Atkin-Smith, Georgia K

AU - Ayre, D Craig

AU - Bach, Jean-Marie

AU - Bachurski, Daniel

AU - Baharvand, Hossein

AU - Balaj, Leonora

AU - Baldacchino, Shawn

AU - Bauer, Natalie N

AU - Baxter, Amy A

AU - Bebawy, Mary

AU - Beckham, Carla

AU - Bedina Zavec, Apolonija

AU - Benmoussa, Abderrahim

AU - Berardi, Anna C

AU - Bergese, Paolo

AU - Bielska, Ewa

AU - Blenkiron, Cherie

AU - Bobis-Wozowicz, Sylwia

AU - Boilard, Eric

AU - Boireau, Wilfrid

AU - Bongiovanni, Antonella

AU - Borràs, Francesc E

AU - Bosch, Steffi

AU - Boulanger, Chantal M

AU - Breakefield, Xandra

AU - Breglio, Andrew M

AU - Brennan, Meadhbh Á

AU - Brigstock, David R

AU - Brisson, Alain

AU - Broekman, Marike Ld

AU - Bromberg, Jacqueline F

AU - Bryl-Górecka, Paulina

AU - Buch, Shilpa

AU - Buck, Amy H

AU - Burger, Dylan

AU - Busatto, Sara

AU - Buschmann, Dominik

AU - Jørgensen, Malene Møller

AU - Jacobsen, Søren

AU - Nejsum, Peter

AU - Østergaard, Ole

AU - Xu, Jing

N1 - Position Paper

PY - 2018

Y1 - 2018

N2 - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

AB - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

U2 - 10.1080/20013078.2018.1535750

DO - 10.1080/20013078.2018.1535750

M3 - Journal article

C2 - 30637094

VL - 7

SP - 1

EP - 47

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

SN - 2001-3078

IS - 1

M1 - 1535750

ER -