MicroRNA expression in the adult mouse central nervous system.
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MicroRNA expression in the adult mouse central nervous system. / Bak, Mads; Silahtaroglu, Asli; Møller, Morten; Christensen, Mette; Rath, Martin F; Skryabin, Boris; Tommerup, Niels; Kauppinen, Sakari.
I: RNA: A publication of the RNA Society, Bind 14, Nr. 3, 2008, s. 432-44.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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T1 - MicroRNA expression in the adult mouse central nervous system.
AU - Bak, Mads
AU - Silahtaroglu, Asli
AU - Møller, Morten
AU - Christensen, Mette
AU - Rath, Martin F
AU - Skryabin, Boris
AU - Tommerup, Niels
AU - Kauppinen, Sakari
N1 - Keywords: Animals; Central Nervous System; Gene Expression; Gene Expression Profiling; In Situ Hybridization; Male; Mice; Mice, Inbred BALB C; MicroRNAs; Reverse Transcriptase Polymerase Chain Reaction; Species Specificity; Tissue Distribution; Zebrafish
PY - 2008
Y1 - 2008
N2 - MicroRNAs are approximately 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3'-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system. Udgivelsesdato: 2008-Mar
AB - MicroRNAs are approximately 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3'-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system. Udgivelsesdato: 2008-Mar
U2 - 10.1261/rna.783108
DO - 10.1261/rna.783108
M3 - Journal article
C2 - 18230762
VL - 14
SP - 432
EP - 444
JO - RNA
JF - RNA
SN - 1355-8382
IS - 3
ER -
ID: 3702652