MicroRNA expression in the adult mouse central nervous system.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

MicroRNA expression in the adult mouse central nervous system. / Bak, Mads; Silahtaroglu, Asli; Møller, Morten; Christensen, Mette; Rath, Martin F; Skryabin, Boris; Tommerup, Niels; Kauppinen, Sakari.

I: RNA: A publication of the RNA Society, Bind 14, Nr. 3, 2008, s. 432-44.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bak, M, Silahtaroglu, A, Møller, M, Christensen, M, Rath, MF, Skryabin, B, Tommerup, N & Kauppinen, S 2008, 'MicroRNA expression in the adult mouse central nervous system.', RNA: A publication of the RNA Society, bind 14, nr. 3, s. 432-44. https://doi.org/10.1261/rna.783108

APA

Bak, M., Silahtaroglu, A., Møller, M., Christensen, M., Rath, M. F., Skryabin, B., ... Kauppinen, S. (2008). MicroRNA expression in the adult mouse central nervous system. RNA: A publication of the RNA Society, 14(3), 432-44. https://doi.org/10.1261/rna.783108

Vancouver

Bak M, Silahtaroglu A, Møller M, Christensen M, Rath MF, Skryabin B o.a. MicroRNA expression in the adult mouse central nervous system. RNA: A publication of the RNA Society. 2008;14(3):432-44. https://doi.org/10.1261/rna.783108

Author

Bak, Mads ; Silahtaroglu, Asli ; Møller, Morten ; Christensen, Mette ; Rath, Martin F ; Skryabin, Boris ; Tommerup, Niels ; Kauppinen, Sakari. / MicroRNA expression in the adult mouse central nervous system. I: RNA: A publication of the RNA Society. 2008 ; Bind 14, Nr. 3. s. 432-44.

Bibtex

@article{e9044fb00c4a11ddbee902004c4f4f50,
title = "MicroRNA expression in the adult mouse central nervous system.",
abstract = "MicroRNAs are approximately 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3'-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50{\%} of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100{\%} conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system. Udgivelsesdato: 2008-Mar",
author = "Mads Bak and Asli Silahtaroglu and Morten M{\o}ller and Mette Christensen and Rath, {Martin F} and Boris Skryabin and Niels Tommerup and Sakari Kauppinen",
note = "Keywords: Animals; Central Nervous System; Gene Expression; Gene Expression Profiling; In Situ Hybridization; Male; Mice; Mice, Inbred BALB C; MicroRNAs; Reverse Transcriptase Polymerase Chain Reaction; Species Specificity; Tissue Distribution; Zebrafish",
year = "2008",
doi = "10.1261/rna.783108",
language = "English",
volume = "14",
pages = "432--44",
journal = "R N A",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "3",

}

RIS

TY - JOUR

T1 - MicroRNA expression in the adult mouse central nervous system.

AU - Bak, Mads

AU - Silahtaroglu, Asli

AU - Møller, Morten

AU - Christensen, Mette

AU - Rath, Martin F

AU - Skryabin, Boris

AU - Tommerup, Niels

AU - Kauppinen, Sakari

N1 - Keywords: Animals; Central Nervous System; Gene Expression; Gene Expression Profiling; In Situ Hybridization; Male; Mice; Mice, Inbred BALB C; MicroRNAs; Reverse Transcriptase Polymerase Chain Reaction; Species Specificity; Tissue Distribution; Zebrafish

PY - 2008

Y1 - 2008

N2 - MicroRNAs are approximately 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3'-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system. Udgivelsesdato: 2008-Mar

AB - MicroRNAs are approximately 22 nucleotide endogenous noncoding RNAs that post-transcriptionally repress expression of protein-coding genes by base-pairing with the 3'-untranslated regions of the target mRNAs. We present here an inventory of miRNA expression profiles from 13 neuroanatomically distinct areas of the adult mouse central nervous system (CNS). Microarray profiling in combination with real-time RT-PCR and LNA (locked nucleic acid)-based in situ hybridization uncovered 44 miRNAs displaying more than threefold enrichment in the spinal cord, cerebellum, medulla oblongata, pons, hypothalamus, hippocampus, neocortex, olfactory bulb, eye, and pituitary gland. These findings suggest that a large number of mouse CNS-expressed miRNAs may be associated with specific functions within these regions. Notably, more than 50% of the identified mouse CNS-enriched miRNAs showed different expression patterns compared to those reported in zebrafish, although the mature miRNA sequences are nearly 100% conserved between the two vertebrate species. The inventory of miRNA profiles in the adult mouse CNS presented here provides an important step toward further elucidation of miRNA function and miRNA-related gene regulatory networks in the mammalian central nervous system. Udgivelsesdato: 2008-Mar

U2 - 10.1261/rna.783108

DO - 10.1261/rna.783108

M3 - Journal article

C2 - 18230762

VL - 14

SP - 432

EP - 444

JO - R N A

JF - R N A

SN - 1355-8382

IS - 3

ER -

ID: 3702652