Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling. / Maimets, Martti; Pedersen, Marianne Terndrup; Guiu, Jordi; Dreier, Jes; Thodberg, Malte; Antoku, Yasuko; Schweiger, Pawel J; Rib, Leonor; Bressan, Raul Bardini; Miao, Yi; Garcia, K. Christopher; Sandelin, Albin; Serup, Palle; Jensen, Kim B.
I: Nature Communications, Bind 13, 715, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Mesenchymal-epithelial crosstalk shapes intestinal regionalisation via Wnt and Shh signalling
AU - Maimets, Martti
AU - Pedersen, Marianne Terndrup
AU - Guiu, Jordi
AU - Dreier, Jes
AU - Thodberg, Malte
AU - Antoku, Yasuko
AU - Schweiger, Pawel J
AU - Rib, Leonor
AU - Bressan, Raul Bardini
AU - Miao, Yi
AU - Garcia, K. Christopher
AU - Sandelin, Albin
AU - Serup, Palle
AU - Jensen, Kim B
N1 - © 2022. The Author(s).
PY - 2022
Y1 - 2022
N2 - Organs are anatomically compartmentalised to cater for specialised functions. In the small intestine (SI), regionalisation enables sequential processing of food and nutrient absorption. While several studies indicate the critical importance of non-epithelial cells during development and homeostasis, the extent to which these cells contribute to regionalisation during morphogenesis remains unexplored. Here, we identify a mesenchymal-epithelial crosstalk that shapes the developing SI during late morphogenesis. We find that subepithelial mesenchymal cells are characterised by gradients of factors supporting Wnt signalling and stimulate epithelial growth in vitro. Such a gradient impacts epithelial gene expression and regional villus formation along the anterior-posterior axis of the SI. Notably, we further provide evidence that Wnt signalling directly regulates epithelial expression of Sonic Hedgehog (SHH), which, in turn, acts on mesenchymal cells to drive villi formation. Taken together our results uncover a mechanistic link between Wnt and Hedgehog signalling across different cellular compartments that is central for anterior-posterior regionalisation and correct formation of the SI.
AB - Organs are anatomically compartmentalised to cater for specialised functions. In the small intestine (SI), regionalisation enables sequential processing of food and nutrient absorption. While several studies indicate the critical importance of non-epithelial cells during development and homeostasis, the extent to which these cells contribute to regionalisation during morphogenesis remains unexplored. Here, we identify a mesenchymal-epithelial crosstalk that shapes the developing SI during late morphogenesis. We find that subepithelial mesenchymal cells are characterised by gradients of factors supporting Wnt signalling and stimulate epithelial growth in vitro. Such a gradient impacts epithelial gene expression and regional villus formation along the anterior-posterior axis of the SI. Notably, we further provide evidence that Wnt signalling directly regulates epithelial expression of Sonic Hedgehog (SHH), which, in turn, acts on mesenchymal cells to drive villi formation. Taken together our results uncover a mechanistic link between Wnt and Hedgehog signalling across different cellular compartments that is central for anterior-posterior regionalisation and correct formation of the SI.
U2 - 10.1038/s41467-022-28369-7
DO - 10.1038/s41467-022-28369-7
M3 - Journal article
C2 - 35132078
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 715
ER -
ID: 291942979