Mechanistic studies on the flavin: NADH reductase (PrnF) from Pseudomonas fluorescens involved in arylamine oxygenation

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  • Manish Kumar Tiwari
  • Raushan Kumar Singh
  • Jung-Kul Lee
  • Huimin Zhao
We report the mechanistic studies of a FAD:NADH reductase (PrnF) involved in arylamine oxygenation. PrnF catalyzes the reduction of FAD via a sequential ordered bi-bi mechanism with NADH as the first substrate to bind and FADH2 as the first product to be released. The residues Asp145 and His146 are proposed as catalytic acid/base residues for PrnF based on pH profile and molecular dynamics simulation studies. These studies provide the first detailed account of the mechanism of the flavin reductase involved in arylamine oxygenation.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
ISSN0960-894X
StatusUdgivet - 2012

ID: 229903528