Localization and function of ATP-sensitive potassium channels in human skeletal muscle
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Localization and function of ATP-sensitive potassium channels in human skeletal muscle. / Nielsen, Jens Jung; Kristensen, Michael; Hellsten, Ylva; Bangsbo, Jens; Juel, Carsten.
I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 284, Nr. 2, 2003, s. R558-R563.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Localization and function of ATP-sensitive potassium channels in human skeletal muscle
AU - Nielsen, Jens Jung
AU - Kristensen, Michael
AU - Hellsten, Ylva
AU - Bangsbo, Jens
AU - Juel, Carsten
N1 - Keywords: Adenosine Triphosphate; Adult; Animals; Blotting, Western; Cell Membrane; Exercise; Glyburide; Humans; Immunohistochemistry; Ion Channel Gating; Male; Muscle, Skeletal; Potassium; Potassium Channels; Rats; Time Factors
PY - 2003
Y1 - 2003
N2 - The present study investigated the localization of ATP-sensitive K+ (KATP) channels in human skeletal muscle and the functional importance of these channels for human muscle K+ distribution at rest and during muscle activity. Membrane fractionation based on the giant vesicle technique or the sucrose-gradient technique in combination with Western blotting demonstrated that the KATP channels are mainly located in the sarcolemma. This localization was confirmed by immunohistochemical measurements. With the microdialysis technique, it was demonstrated that local application of the KATP channel inhibitor glibenclamide reduced (P < 0.05) interstitial K+ at rest from approximately 4.5 to 4.0 mM, whereas the concentration in the control leg remained constant. Glibenclamide had no effect on the interstitial K+ accumulation during knee-extensor exercise at a power output of 60 W. In contrast to in vitro conditions, the present study demonstrated that under in vivo conditions the KATP channels are active at rest and contribute to the accumulation of interstitial K+.
AB - The present study investigated the localization of ATP-sensitive K+ (KATP) channels in human skeletal muscle and the functional importance of these channels for human muscle K+ distribution at rest and during muscle activity. Membrane fractionation based on the giant vesicle technique or the sucrose-gradient technique in combination with Western blotting demonstrated that the KATP channels are mainly located in the sarcolemma. This localization was confirmed by immunohistochemical measurements. With the microdialysis technique, it was demonstrated that local application of the KATP channel inhibitor glibenclamide reduced (P < 0.05) interstitial K+ at rest from approximately 4.5 to 4.0 mM, whereas the concentration in the control leg remained constant. Glibenclamide had no effect on the interstitial K+ accumulation during knee-extensor exercise at a power output of 60 W. In contrast to in vitro conditions, the present study demonstrated that under in vivo conditions the KATP channels are active at rest and contribute to the accumulation of interstitial K+.
U2 - 10.1152/ajpregu.00303.2002
DO - 10.1152/ajpregu.00303.2002
M3 - Journal article
C2 - 12388475
VL - 284
SP - R558-R563
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 2
ER -
ID: 17521698