Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
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Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats. / Drinkwater, Rosie; Jucker, Tommaso; Potter, Joshua H. T.; Swinfield, Tom; Coomes, David A.; Slade, Eleanor M.; Gilbert, M. Thomas P.; Lewis, Owen T.; Bernard, Henry; Struebig, Matthew J.; Clare, Elizabeth L.; Rossiter, Stephen J.
I: Molecular Ecology, Bind 30, Nr. 13, 2021, s. 3299-3312.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
AU - Drinkwater, Rosie
AU - Jucker, Tommaso
AU - Potter, Joshua H. T.
AU - Swinfield, Tom
AU - Coomes, David A.
AU - Slade, Eleanor M.
AU - Gilbert, M. Thomas P.
AU - Lewis, Owen T.
AU - Bernard, Henry
AU - Struebig, Matthew J.
AU - Clare, Elizabeth L.
AU - Rossiter, Stephen J.
PY - 2021
Y1 - 2021
N2 - The application of metabarcoding to environmental and invertebrate-derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid land-use change for agriculture means there is a growing need to understand the conservation value of the remaining mosaic and degraded landscapes. Here we use iDNA from blood-feeding leeches (Haemadipsa picta) to assess differences in mammalian diversity across a gradient of forest degradation in Sabah, Malaysian Borneo. We screened 557 individual leeches for mammal DNA by targeting fragments of the 16S rRNA gene and detected 14 mammalian genera. We recorded lower mammal diversity in the most heavily degraded forest compared to higher quality twice logged forest. Although the accumulation curves of diversity estimates were comparable across these habitat types, diversity was higher in twice logged forest, with more taxa of conservation concern. In addition, our analysis revealed differences between the community recorded in the heavily logged forest and that of the twice logged forest. By revealing differences in mammal diversity across a human-modified tropical landscape, our study demonstrates the value of iDNA as a noninvasive biomonitoring approach in conservation assessments.
AB - The application of metabarcoding to environmental and invertebrate-derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid land-use change for agriculture means there is a growing need to understand the conservation value of the remaining mosaic and degraded landscapes. Here we use iDNA from blood-feeding leeches (Haemadipsa picta) to assess differences in mammalian diversity across a gradient of forest degradation in Sabah, Malaysian Borneo. We screened 557 individual leeches for mammal DNA by targeting fragments of the 16S rRNA gene and detected 14 mammalian genera. We recorded lower mammal diversity in the most heavily degraded forest compared to higher quality twice logged forest. Although the accumulation curves of diversity estimates were comparable across these habitat types, diversity was higher in twice logged forest, with more taxa of conservation concern. In addition, our analysis revealed differences between the community recorded in the heavily logged forest and that of the twice logged forest. By revealing differences in mammal diversity across a human-modified tropical landscape, our study demonstrates the value of iDNA as a noninvasive biomonitoring approach in conservation assessments.
KW - biodiversity
KW - Borneo
KW - Haemadipsidae
KW - invertebrate-derived DNA
KW - land-use change
KW - molecular biomonitoring
U2 - 10.1111/mec.15724
DO - 10.1111/mec.15724
M3 - Journal article
C2 - 33171014
AN - SCOPUS:85096862764
VL - 30
SP - 3299
EP - 3312
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 13
ER -
ID: 253184711