TY - JOUR

T1 - Labeling and label free shotgun proteomics approaches to characterize muscle tissue from farmed and wild gilthead sea bream (Sparus aurata)

AU - Piovesana, Susy

AU - Capriotti, Anna Laura

AU - Caruso, Giuseppe

AU - Cavaliere, Chiara

AU - La Barbera, Giorgia

AU - Zenezini Chiozzi, Riccardo

AU - Laganà, Aldo

N1 - (Ekstern)

PY - 2016

Y1 - 2016

N2 - The proteome characterization of fish muscle tissues, together with the relative expression of each individual protein, provides knowledge on the biochemical response of the organisms and allows to assess the effect of different types of feeding, growth site and nutritional quality of the investigated species. This type of study is usually performed by gel-based proteomics approaches, however shotgun proteomics can serve as well, reducing analysis time and improving sample high-throughput. In this work, a shotgun proteomics method was thus developed and then applied to the characterization of gilthead sea bream edible muscle. The sarcoplasmic protein fraction was extracted, in-solution digested by trypsin and finally analyzed by nanoHPLC high resolution tandem mass spectrometry. Two different quantification strategies were also tested. One was based on chemical dimethyl labeling and the other one on label free quantification. A comparison between these two analytical workflows was performed, to evaluate their individual performance in the analysis of fish samples and assess the differences induced by farming practice on the final commercial product with respect to wild gilthead sea bream. Quantitative differences were detected, and the most relevant one regarded the common fish allergen parvalbumin, found overexpressed in farmed fish samples.

AB - The proteome characterization of fish muscle tissues, together with the relative expression of each individual protein, provides knowledge on the biochemical response of the organisms and allows to assess the effect of different types of feeding, growth site and nutritional quality of the investigated species. This type of study is usually performed by gel-based proteomics approaches, however shotgun proteomics can serve as well, reducing analysis time and improving sample high-throughput. In this work, a shotgun proteomics method was thus developed and then applied to the characterization of gilthead sea bream edible muscle. The sarcoplasmic protein fraction was extracted, in-solution digested by trypsin and finally analyzed by nanoHPLC high resolution tandem mass spectrometry. Two different quantification strategies were also tested. One was based on chemical dimethyl labeling and the other one on label free quantification. A comparison between these two analytical workflows was performed, to evaluate their individual performance in the analysis of fish samples and assess the differences induced by farming practice on the final commercial product with respect to wild gilthead sea bream. Quantitative differences were detected, and the most relevant one regarded the common fish allergen parvalbumin, found overexpressed in farmed fish samples.

KW - Dimethyl labeling

KW - Food fish proteome

KW - Label free proteomics

KW - Mass spectrometry

KW - Quantification

KW - Shotgun proteomics

UR - http://www.scopus.com/inward/record.url?scp=84966495637&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2015.07.049

DO - 10.1016/j.chroma.2015.07.049

M3 - Journal article

C2 - 26233254

AN - SCOPUS:84966495637

VL - 1428

SP - 193

EP - 201

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0301-4770

ER -