Isolation of vancomycin resistant Enterococcus faecium from food
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Isolation of vancomycin resistant Enterococcus faecium from food. / Wegener, Henrik Caspar; Madsen, Mogens; Nielsen, Niels; Aarestrup, Frank Møller.
I: International Journal of Food Microbiology, Bind 35, Nr. 1, 18.03.1997, s. 57-66.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Isolation of vancomycin resistant Enterococcus faecium from food
AU - Wegener, Henrik Caspar
AU - Madsen, Mogens
AU - Nielsen, Niels
AU - Aarestrup, Frank Møller
PY - 1997/3/18
Y1 - 1997/3/18
N2 - In a survey of vancomycin resistant Enterococcus faecium (VREF) in Danish meat products, VREF could be detected in 16% of 160 samples of broilers collected at slaughterhouses and in 15% of 26 samples of pork collected from the retail trade. VREF were isolated by enrichment for 24 h in nutrient broth supplemented with vancomycin 150 μg/ml) prior to plating on Slanetz and Bartley agar. Using direct plating on Slanetz and Bartley agar, VREF could be isolated from only 1.7% of 540 samples of broilers from slaughterhouses and 2.2% of 90 samples of broilers from retail outlets. VREF was not detected in 124 samples of pork and 128 samples of beef from retail outlets by the direct plating method. An additional enrichment step in nutrient broth supplemented with vancomycin enhanced the detection rate of VREF by approximately three times compared to the direct plating method when investigating the same 160 samples of broilers by the two methods. The implications and public health aspects of VREF in food is discussed.
AB - In a survey of vancomycin resistant Enterococcus faecium (VREF) in Danish meat products, VREF could be detected in 16% of 160 samples of broilers collected at slaughterhouses and in 15% of 26 samples of pork collected from the retail trade. VREF were isolated by enrichment for 24 h in nutrient broth supplemented with vancomycin 150 μg/ml) prior to plating on Slanetz and Bartley agar. Using direct plating on Slanetz and Bartley agar, VREF could be isolated from only 1.7% of 540 samples of broilers from slaughterhouses and 2.2% of 90 samples of broilers from retail outlets. VREF was not detected in 124 samples of pork and 128 samples of beef from retail outlets by the direct plating method. An additional enrichment step in nutrient broth supplemented with vancomycin enhanced the detection rate of VREF by approximately three times compared to the direct plating method when investigating the same 160 samples of broilers by the two methods. The implications and public health aspects of VREF in food is discussed.
KW - Beef
KW - Broilers
KW - Detection
KW - E. faecium
KW - Food
KW - Pork
KW - Vancomycin resistance
UR - http://www.scopus.com/inward/record.url?scp=0030934693&partnerID=8YFLogxK
U2 - 10.1016/S0168-1605(96)01221-4
DO - 10.1016/S0168-1605(96)01221-4
M3 - Journal article
C2 - 9081226
AN - SCOPUS:0030934693
VL - 35
SP - 57
EP - 66
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 1
ER -
ID: 228688459