Ionized calcium-binding adaptor molecule 1 positive macrophages and HO-1 up-regulation in intestinal muscularis resident macrophages
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- Ionized calcium-binding adaptor molecule 1 positive macrophages and HO-1 up-regulation in intestinal muscularis resident macrophages
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Small intestinal muscularis externa macrophages have been associated with interstitial cells of Cajal (ICC). They have been proposed to play various roles in motility disorders and to take part in a microbiota-driven regulation of gastrointestinal motility. Our objective was to understand the reaction of resident macrophages of the musculature to a pro-inflammatory stimulator, lipopolysaccharide (LPS). Mice were injected with LPS or saline and sacrificed after 6 hours. Whole mounts were stained with antibodies toward CD169, ionized calcium-binding adaptor molecule 1 (iba1) (microglial/macrophage marker) and heme oxygenase-1 (HO-1). Cell densities were measured using unbiased stereology.
RESULTS: iba1(pos) cells showed an overall higher density than CD169(pos) and HO-1(pos) cells. Most HO-1(pos) and iba1(pos) cells were positive for CD 169 in serosa and at Auerbach's plexus (AP). At the deep muscular plexus, mainly iba1(pos) cells were present, and were mostly CD169(neg) ; a few HO-1(pos) cells were present.
CONCLUSIONS: A new subset of resident macrophages in the intestinal muscularis externa was discovered, identified as iba1(pos) CD169(neg) . HO-1 is constitutively present in most macrophages in serosa and at AP, suggesting a M2 phenotype. LPS-treatment results in an up-regulation of HO-1(pos) /CD169(neg) cells in serosa and at AP. This article is protected by copyright. All rights reserved.
|Status||Udgivet - jun. 2017|
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