Inhibition of lpmos by fermented persimmon juice

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Standard

Inhibition of lpmos by fermented persimmon juice. / Tokin, Radina; Ipsen, Johan Ørskov; Poojary, Mahesha M.; Jensen, Poul Erik; Olsson, Lisbeth; Johansen, Katja Salomon.

I: Biomolecules, Bind 11, Nr. 12, 1890, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Tokin, R, Ipsen, JØ, Poojary, MM, Jensen, PE, Olsson, L & Johansen, KS 2021, 'Inhibition of lpmos by fermented persimmon juice', Biomolecules, bind 11, nr. 12, 1890. https://doi.org/10.3390/biom11121890

APA

Tokin, R., Ipsen, J. Ø., Poojary, M. M., Jensen, P. E., Olsson, L., & Johansen, K. S. (2021). Inhibition of lpmos by fermented persimmon juice. Biomolecules, 11(12), [1890]. https://doi.org/10.3390/biom11121890

Vancouver

Tokin R, Ipsen JØ, Poojary MM, Jensen PE, Olsson L, Johansen KS. Inhibition of lpmos by fermented persimmon juice. Biomolecules. 2021;11(12). 1890. https://doi.org/10.3390/biom11121890

Author

Tokin, Radina ; Ipsen, Johan Ørskov ; Poojary, Mahesha M. ; Jensen, Poul Erik ; Olsson, Lisbeth ; Johansen, Katja Salomon. / Inhibition of lpmos by fermented persimmon juice. I: Biomolecules. 2021 ; Bind 11, Nr. 12.

Bibtex

@article{d133f401f4cb46219f2ac6163e499dd3,
title = "Inhibition of lpmos by fermented persimmon juice",
abstract = "Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.",
keywords = "Cellulase, Inhibition, Kakishibu, LPMO, PEG, Tannins",
author = "Radina Tokin and Ipsen, {Johan {\O}rskov} and Poojary, {Mahesha M.} and Jensen, {Poul Erik} and Lisbeth Olsson and Johansen, {Katja Salomon}",
note = "Funding Information: Funding: This research was funded by The Novo Nordisk Foundation (Grant number NNF17SA0027704 to Katja Salomon Johansen). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
doi = "10.3390/biom11121890",
language = "English",
volume = "11",
journal = "Biomolecules",
issn = "2218-273X",
publisher = "MDPI",
number = "12",

}

RIS

TY - JOUR

T1 - Inhibition of lpmos by fermented persimmon juice

AU - Tokin, Radina

AU - Ipsen, Johan Ørskov

AU - Poojary, Mahesha M.

AU - Jensen, Poul Erik

AU - Olsson, Lisbeth

AU - Johansen, Katja Salomon

N1 - Funding Information: Funding: This research was funded by The Novo Nordisk Foundation (Grant number NNF17SA0027704 to Katja Salomon Johansen). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021

Y1 - 2021

N2 - Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.

AB - Fermented persimmon juice, Kakishibu, has traditionally been used for wood and paper protection. This protective effect stems at least partially from inhibition of microbial cellulose degrading enzymes. The inhibitory effect of Kakishibu on lytic polysaccharide monooxygenases (LPMOs) and on a cocktail of cellulose hydrolases was studied, using three different cellulosic substrates. Dose dependent inhibition of LPMO activity by a commercial Kakishibu product was assessed for the well-characterized LPMO from Thermoascus aurantiacus TaAA9A, and the inhibitory effect was confirmed on five additional microbial LPMOs. The model tannin compound, tannic acid exhibited a similar inhibitory effect on TaAA9A as Kakishibu. It was further shown that both polyethylene glycol and tannase can alleviate the inhibitory effect of Kakishibu and tannic acid, indicating a likely mechanism of inhibition caused by unspecific tannin–protein interactions.

KW - Cellulase

KW - Inhibition

KW - Kakishibu

KW - LPMO

KW - PEG

KW - Tannins

U2 - 10.3390/biom11121890

DO - 10.3390/biom11121890

M3 - Journal article

C2 - 34944533

AN - SCOPUS:85121154854

VL - 11

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 12

M1 - 1890

ER -

ID: 288118332