Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells. / Urizar, Adriana Ibarra; Friberg, Josefine; Christensen, Dan Ploug; Christensen, Gitte Lund; Billestrup, Nils.

I: Journal of Interferon & Cytokine Research, Bind 36, Nr. 1, 01.01.2016, s. 20-29.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Urizar, AI, Friberg, J, Christensen, DP, Christensen, GL & Billestrup, N 2016, 'Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells', Journal of Interferon & Cytokine Research, bind 36, nr. 1, s. 20-29. https://doi.org/10.1089/jir.2014.0199

APA

Urizar, A. I., Friberg, J., Christensen, D. P., Christensen, G. L., & Billestrup, N. (2016). Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells. Journal of Interferon & Cytokine Research, 36(1), 20-29. https://doi.org/10.1089/jir.2014.0199

Vancouver

Urizar AI, Friberg J, Christensen DP, Christensen GL, Billestrup N. Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells. Journal of Interferon & Cytokine Research. 2016 jan. 1;36(1):20-29. https://doi.org/10.1089/jir.2014.0199

Author

Urizar, Adriana Ibarra ; Friberg, Josefine ; Christensen, Dan Ploug ; Christensen, Gitte Lund ; Billestrup, Nils. / Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells. I: Journal of Interferon & Cytokine Research. 2016 ; Bind 36, Nr. 1. s. 20-29.

Bibtex

@article{61f5dd5a8e004fd099825aa3babacc6f,
title = "Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells",
abstract = "The proinflammatory cytokines interleukin-1 beta (IL-1β) and interferon gamma (IFN-γ) play important roles in the progressive loss of beta-cell mass and function during development of both type 1 and type 2 diabetes. We have recently showed that bone morphogenetic protein (BMP)-2 and -4 are expressed in pancreatic islets and inhibit beta-cell growth and function. In this study, we describe that IL-1β and IFN-γ induce the expression of BMP-2 suggesting a possible role for BMP-2 in mediating the effects of IL-1β and IFN-γ on beta-cell apoptosis and dysfunction. IL-1β increased BMP-2 mRNA levels 6- and 3-fold in isolated islets of Langerhans from neonatal rat and human. Downstream target genes of the BMP pathway were also increased by cytokine treatment and could be reversed by neutralization of endogenous BMP activity. Nuclear factor kappa B- (NFκB) binding sites were identified in the rat BMP-2 promoter, and reporter assays verified the role of NFκB in cytokine-induced BMP-2 expression. Electrophoretic mobility shift assay and chromatin immunoprecipitation assays confirmed NFκB binding to BMP-2 promoter upon IL-1β stimulation in beta cells. In conclusion, we suggest that NFκB stimulates BMP-2 mRNA expression in rat and human beta cells upon cytokine exposure.",
author = "Urizar, {Adriana Ibarra} and Josefine Friberg and Christensen, {Dan Ploug} and Christensen, {Gitte Lund} and Nils Billestrup",
year = "2016",
month = jan,
day = "1",
doi = "10.1089/jir.2014.0199",
language = "English",
volume = "36",
pages = "20--29",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "1",

}

RIS

TY - JOUR

T1 - Inflammatory Cytokines Stimulate Bone Morphogenetic Protein-2 Expression and Release from Pancreatic Beta Cells

AU - Urizar, Adriana Ibarra

AU - Friberg, Josefine

AU - Christensen, Dan Ploug

AU - Christensen, Gitte Lund

AU - Billestrup, Nils

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The proinflammatory cytokines interleukin-1 beta (IL-1β) and interferon gamma (IFN-γ) play important roles in the progressive loss of beta-cell mass and function during development of both type 1 and type 2 diabetes. We have recently showed that bone morphogenetic protein (BMP)-2 and -4 are expressed in pancreatic islets and inhibit beta-cell growth and function. In this study, we describe that IL-1β and IFN-γ induce the expression of BMP-2 suggesting a possible role for BMP-2 in mediating the effects of IL-1β and IFN-γ on beta-cell apoptosis and dysfunction. IL-1β increased BMP-2 mRNA levels 6- and 3-fold in isolated islets of Langerhans from neonatal rat and human. Downstream target genes of the BMP pathway were also increased by cytokine treatment and could be reversed by neutralization of endogenous BMP activity. Nuclear factor kappa B- (NFκB) binding sites were identified in the rat BMP-2 promoter, and reporter assays verified the role of NFκB in cytokine-induced BMP-2 expression. Electrophoretic mobility shift assay and chromatin immunoprecipitation assays confirmed NFκB binding to BMP-2 promoter upon IL-1β stimulation in beta cells. In conclusion, we suggest that NFκB stimulates BMP-2 mRNA expression in rat and human beta cells upon cytokine exposure.

AB - The proinflammatory cytokines interleukin-1 beta (IL-1β) and interferon gamma (IFN-γ) play important roles in the progressive loss of beta-cell mass and function during development of both type 1 and type 2 diabetes. We have recently showed that bone morphogenetic protein (BMP)-2 and -4 are expressed in pancreatic islets and inhibit beta-cell growth and function. In this study, we describe that IL-1β and IFN-γ induce the expression of BMP-2 suggesting a possible role for BMP-2 in mediating the effects of IL-1β and IFN-γ on beta-cell apoptosis and dysfunction. IL-1β increased BMP-2 mRNA levels 6- and 3-fold in isolated islets of Langerhans from neonatal rat and human. Downstream target genes of the BMP pathway were also increased by cytokine treatment and could be reversed by neutralization of endogenous BMP activity. Nuclear factor kappa B- (NFκB) binding sites were identified in the rat BMP-2 promoter, and reporter assays verified the role of NFκB in cytokine-induced BMP-2 expression. Electrophoretic mobility shift assay and chromatin immunoprecipitation assays confirmed NFκB binding to BMP-2 promoter upon IL-1β stimulation in beta cells. In conclusion, we suggest that NFκB stimulates BMP-2 mRNA expression in rat and human beta cells upon cytokine exposure.

U2 - 10.1089/jir.2014.0199

DO - 10.1089/jir.2014.0199

M3 - Journal article

C2 - 26308798

VL - 36

SP - 20

EP - 29

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 1

ER -

ID: 164621137