ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children
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ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children. / Suurbaar, Jennifer; Moussiliou, Azizath; Tahar, Rachida; Olsen, Rebecca W; Adams, Yvonne; Dalgaard, Nanna; Baafour, Eric K; Adukpo, Selorme; Hviid, Lars; Kusi, Kwadwo A; Alao, Jules; Ofori, Michael F; Ndam, Nicaise T; Jensen, Anja R.
I: Scientific Reports, Bind 12, 12994, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children
AU - Suurbaar, Jennifer
AU - Moussiliou, Azizath
AU - Tahar, Rachida
AU - Olsen, Rebecca W
AU - Adams, Yvonne
AU - Dalgaard, Nanna
AU - Baafour, Eric K
AU - Adukpo, Selorme
AU - Hviid, Lars
AU - Kusi, Kwadwo A
AU - Alao, Jules
AU - Ofori, Michael F
AU - Ndam, Nicaise T
AU - Jensen, Anja R
N1 - © 2022. The Author(s).
PY - 2022
Y1 - 2022
N2 - Members of the highly polymorphic Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of infected erythrocytes (IEs) are important virulence factors, which mediate vascular adhesion of IEs via endothelial host receptors and are targets of naturally acquired immunity. The PfEMP1 family can be divided into clinically relevant subgroups, of which some bind intercellular adhesion molecule 1 (ICAM-1). While the acquisition of IgG specific for ICAM-1-binding DBLβ domains is known to differ between PfEMP1 groups, its ability to induce antibody-dependent cellular phagocytosis (ADCP) is unclear. We therefore measured plasma levels of DBLβ-specific IgG, the ability of such IgG to inhibit PfEMP1-binding to ICAM-1, and its ability to opsonize IEs for ADCP, using plasma from Beninese children with severe (SM) or uncomplicated malaria (UM). IgG specific for DBLβ from group A and B ICAM-1-binding PfEMP1 were dominated by IgG1 and IgG3, and were similar in SM and UM. However, levels of plasma IgG inhibiting ICAM-1-binding of group A DBLβ of PFD1235w was significantly higher in children with UM than SM, and acute UM plasma induced a higher ADCP response than acute SM plasma.
AB - Members of the highly polymorphic Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of infected erythrocytes (IEs) are important virulence factors, which mediate vascular adhesion of IEs via endothelial host receptors and are targets of naturally acquired immunity. The PfEMP1 family can be divided into clinically relevant subgroups, of which some bind intercellular adhesion molecule 1 (ICAM-1). While the acquisition of IgG specific for ICAM-1-binding DBLβ domains is known to differ between PfEMP1 groups, its ability to induce antibody-dependent cellular phagocytosis (ADCP) is unclear. We therefore measured plasma levels of DBLβ-specific IgG, the ability of such IgG to inhibit PfEMP1-binding to ICAM-1, and its ability to opsonize IEs for ADCP, using plasma from Beninese children with severe (SM) or uncomplicated malaria (UM). IgG specific for DBLβ from group A and B ICAM-1-binding PfEMP1 were dominated by IgG1 and IgG3, and were similar in SM and UM. However, levels of plasma IgG inhibiting ICAM-1-binding of group A DBLβ of PFD1235w was significantly higher in children with UM than SM, and acute UM plasma induced a higher ADCP response than acute SM plasma.
U2 - 10.1038/s41598-022-16305-0
DO - 10.1038/s41598-022-16305-0
M3 - Journal article
C2 - 35906450
VL - 12
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 12994
ER -
ID: 315202097