High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants

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Standard

High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants. / Kongstad, Kenneth; Wubshet, Sileshi Gizachew; Johannesen, Ane; Kjellerup, Lasse; winther, Anne-Marie Lund; Jäger, Anna; Stærk, Dan.

I: Journal of Agricultural and Food Chemistry, Bind 62, Nr. 24, 2014, s. 5595-5602.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kongstad, K, Wubshet, SG, Johannesen, A, Kjellerup, L, winther, A-ML, Jäger, A & Stærk, D 2014, 'High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants', Journal of Agricultural and Food Chemistry, bind 62, nr. 24, s. 5595-5602. https://doi.org/10.1021/jf501605z

APA

Kongstad, K., Wubshet, S. G., Johannesen, A., Kjellerup, L., winther, A-M. L., Jäger, A., & Stærk, D. (2014). High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants. Journal of Agricultural and Food Chemistry, 62(24), 5595-5602. https://doi.org/10.1021/jf501605z

Vancouver

Kongstad K, Wubshet SG, Johannesen A, Kjellerup L, winther A-ML, Jäger A o.a. High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants. Journal of Agricultural and Food Chemistry. 2014;62(24):5595-5602. https://doi.org/10.1021/jf501605z

Author

Kongstad, Kenneth ; Wubshet, Sileshi Gizachew ; Johannesen, Ane ; Kjellerup, Lasse ; winther, Anne-Marie Lund ; Jäger, Anna ; Stærk, Dan. / High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants. I: Journal of Agricultural and Food Chemistry. 2014 ; Bind 62, Nr. 24. s. 5595-5602.

Bibtex

@article{e6655c610d744e7ea49c628e991e1d9a,
title = "High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants",
abstract = "Crude extracts of 33 plant species were assessed for fungal plasma membrane (PM) H+-ATPase inhibition. This led to identification of 18 extracts showing more than 95% inhibition at a concentration of 7.5 mg/mL and/or a concentration-dependent activity profile. These extracts were selected for semi-high-resolution fungal PM H+-ATPase inhibition screening, and, on the basis of these results, Haplocoelum foliolosum (Hiern) Bullock and Sauvagesia erecta L. were selected for investigation by high-resolution fungal PM H+-ATPase inhibition screening. Structural analysis performed by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR) led to identification of chebulagic acid (1) and tellimagrandin II (2) from H. foliolosum. Preparative-scale isolation of the two metabolites allowed determination of IC50 values for PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. Chebulagic acid and tellimagrandin II are both potent inhibitors of the PM H+-ATPase with inhibitory effect on the growth of S. cerevisiae.",
author = "Kenneth Kongstad and Wubshet, {Sileshi Gizachew} and Ane Johannesen and Lasse Kjellerup and winther, {Anne-Marie Lund} and Anna J{\"a}ger and Dan St{\ae}rk",
year = "2014",
doi = "10.1021/jf501605z",
language = "English",
volume = "62",
pages = "5595--5602",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "24",

}

RIS

TY - JOUR

T1 - High-resolution screening combined with HPLC–HRMS–SPE–NMR for identification of fungal plasma membrane H+-ATPase inhibitors from plants

AU - Kongstad, Kenneth

AU - Wubshet, Sileshi Gizachew

AU - Johannesen, Ane

AU - Kjellerup, Lasse

AU - winther, Anne-Marie Lund

AU - Jäger, Anna

AU - Stærk, Dan

PY - 2014

Y1 - 2014

N2 - Crude extracts of 33 plant species were assessed for fungal plasma membrane (PM) H+-ATPase inhibition. This led to identification of 18 extracts showing more than 95% inhibition at a concentration of 7.5 mg/mL and/or a concentration-dependent activity profile. These extracts were selected for semi-high-resolution fungal PM H+-ATPase inhibition screening, and, on the basis of these results, Haplocoelum foliolosum (Hiern) Bullock and Sauvagesia erecta L. were selected for investigation by high-resolution fungal PM H+-ATPase inhibition screening. Structural analysis performed by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR) led to identification of chebulagic acid (1) and tellimagrandin II (2) from H. foliolosum. Preparative-scale isolation of the two metabolites allowed determination of IC50 values for PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. Chebulagic acid and tellimagrandin II are both potent inhibitors of the PM H+-ATPase with inhibitory effect on the growth of S. cerevisiae.

AB - Crude extracts of 33 plant species were assessed for fungal plasma membrane (PM) H+-ATPase inhibition. This led to identification of 18 extracts showing more than 95% inhibition at a concentration of 7.5 mg/mL and/or a concentration-dependent activity profile. These extracts were selected for semi-high-resolution fungal PM H+-ATPase inhibition screening, and, on the basis of these results, Haplocoelum foliolosum (Hiern) Bullock and Sauvagesia erecta L. were selected for investigation by high-resolution fungal PM H+-ATPase inhibition screening. Structural analysis performed by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR) led to identification of chebulagic acid (1) and tellimagrandin II (2) from H. foliolosum. Preparative-scale isolation of the two metabolites allowed determination of IC50 values for PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. Chebulagic acid and tellimagrandin II are both potent inhibitors of the PM H+-ATPase with inhibitory effect on the growth of S. cerevisiae.

U2 - 10.1021/jf501605z

DO - 10.1021/jf501605z

M3 - Journal article

C2 - 24830509

VL - 62

SP - 5595

EP - 5602

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 24

ER -

ID: 110838170