TY - JOUR

T1 - Hepatic mitochondrial oxidative phosphorylation is normal in obese patients with and without type 2 diabetes

AU - Lund, Michael Taulo

AU - Kristensen, Marianne Dalsgaard

AU - Hansen, Merethe

AU - Tveskov, Louise

AU - Floyd, Andrea Karen

AU - Støckel, Mikael

AU - Vainer, Ben

AU - Poulsen, Steen Seier

AU - Helge, Jørn Wulff

AU - Prats Gavalda, Clara

AU - Dela, Flemming

N1 - This article is protected by copyright. All rights reserved.

PY - 2016/4/6

Y1 - 2016/4/6

N2 - INTRODUCTION: Obese patients with (T2DM) and without (OB) type 2 diabetes are characterized by high hepatic lipid content and hepatic insulin resistance. This may be linked to impaired hepatic mitochondrial oxidative phosphorylation (OXPHOS) capacity. The aim of the present study was to investigate and compare hepatic mitochondrial OXPHOS capacity in T2DM, OB and non-obese controls (CON).METHODS: Seventeen obese patients (nine OB and eight T2DM) and six CON patients had perioperative liver biopsies taken. Samples were divided in three parts to measure 1: Complex I, II and IV linked respiration, 2: Citrate synthase (CS) activity and 3: Lipid droplet (LD) size and area (% of total tissue area filled by LDs).RESULTS: State 3 respiration of complex I, II and IV and the CS activity did not differ in OB, T2DM and CON. LD size was significantly higher in T2DM compared with CON and LD area tended (p = 0.10) to be higher in T2DM and OB compared with CON.CONCLUSION: The present findings indicate that hepatic OXPHOS capacity is not different in patients with markedly different weight and glycemic control. Furthermore, the results do not support impaired hepatic mitochondrial respiratory capacity playing a major role in the development of obesity-induced type 2 diabetes. This article is protected by copyright. All rights reserved.

AB - INTRODUCTION: Obese patients with (T2DM) and without (OB) type 2 diabetes are characterized by high hepatic lipid content and hepatic insulin resistance. This may be linked to impaired hepatic mitochondrial oxidative phosphorylation (OXPHOS) capacity. The aim of the present study was to investigate and compare hepatic mitochondrial OXPHOS capacity in T2DM, OB and non-obese controls (CON).METHODS: Seventeen obese patients (nine OB and eight T2DM) and six CON patients had perioperative liver biopsies taken. Samples were divided in three parts to measure 1: Complex I, II and IV linked respiration, 2: Citrate synthase (CS) activity and 3: Lipid droplet (LD) size and area (% of total tissue area filled by LDs).RESULTS: State 3 respiration of complex I, II and IV and the CS activity did not differ in OB, T2DM and CON. LD size was significantly higher in T2DM compared with CON and LD area tended (p = 0.10) to be higher in T2DM and OB compared with CON.CONCLUSION: The present findings indicate that hepatic OXPHOS capacity is not different in patients with markedly different weight and glycemic control. Furthermore, the results do not support impaired hepatic mitochondrial respiratory capacity playing a major role in the development of obesity-induced type 2 diabetes. This article is protected by copyright. All rights reserved.

U2 - 10.1113/JP272105

DO - 10.1113/JP272105

M3 - Journal article

C2 - 27060482

VL - 594

SP - 4351

EP - 4358

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 15

ER -