GLUT-4 content in plasma membrane of muscle from patients with non-insulin-dependent diabetes mellitus
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GLUT-4 content in plasma membrane of muscle from patients with non-insulin-dependent diabetes mellitus. / Lund, S; Vestergaard, H; Andersen, P H; Schmitz, O; Gøtzsche, L B; Pedersen, O.
I: American Journal of Physiology (Consolidated), Bind 265, Nr. 6 Pt 1, 12.1993, s. E889-97.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - GLUT-4 content in plasma membrane of muscle from patients with non-insulin-dependent diabetes mellitus
AU - Lund, S
AU - Vestergaard, H
AU - Andersen, P H
AU - Schmitz, O
AU - Gøtzsche, L B
AU - Pedersen, O
PY - 1993/12
Y1 - 1993/12
N2 - The abundance of GLUT-4 protein in both total crude membrane and plasma membrane fractions of vastus lateralis muscle from 13 obese non-insulin-dependent diabetes mellitus (NIDDM) patients and 14 healthy subjects were examined in the fasting state and after supraphysiological hyperinsulinemia. In the basal state the immunoreactive mass of GLUT-4 protein both in the crude membrane preparation and in the plasma membrane fraction was similar in NIDDM patients and control subjects. Moreover, in vivo insulin exposure neither for 30 min nor for 4 h had any impact on the content of GLUT-4 protein in plasma membranes. With the use of the same methodology, antibody, and achieving the same degree of plasma membrane purification and recovery, we found, however, that intraperitoneal administration of insulin to 7-wk-old rats within 30 min increased the content of GLUT-4 protein more than twofold (P < 0.01) in the plasma membrane from red gastrocnemius and soleus muscle. In conclusion, when the subcellular fractionation method was applied to human muscle biopsies taken in the basal state, no difference could be found in the plasma membrane content of immunoreactive GLUT-4 protein between NIDDM patients and normal subjects. With this technique, we were unable to show evidence for a regulatory effect of insulin on the plasma membrane level of GLUT-4 protein in human muscle.
AB - The abundance of GLUT-4 protein in both total crude membrane and plasma membrane fractions of vastus lateralis muscle from 13 obese non-insulin-dependent diabetes mellitus (NIDDM) patients and 14 healthy subjects were examined in the fasting state and after supraphysiological hyperinsulinemia. In the basal state the immunoreactive mass of GLUT-4 protein both in the crude membrane preparation and in the plasma membrane fraction was similar in NIDDM patients and control subjects. Moreover, in vivo insulin exposure neither for 30 min nor for 4 h had any impact on the content of GLUT-4 protein in plasma membranes. With the use of the same methodology, antibody, and achieving the same degree of plasma membrane purification and recovery, we found, however, that intraperitoneal administration of insulin to 7-wk-old rats within 30 min increased the content of GLUT-4 protein more than twofold (P < 0.01) in the plasma membrane from red gastrocnemius and soleus muscle. In conclusion, when the subcellular fractionation method was applied to human muscle biopsies taken in the basal state, no difference could be found in the plasma membrane content of immunoreactive GLUT-4 protein between NIDDM patients and normal subjects. With this technique, we were unable to show evidence for a regulatory effect of insulin on the plasma membrane level of GLUT-4 protein in human muscle.
KW - 4-Nitrophenylphosphatase
KW - Animals
KW - Blood Glucose
KW - Blotting, Western
KW - C-Peptide
KW - Cell Membrane
KW - Diabetes Mellitus, Type 2
KW - Fatty Acids, Nonesterified
KW - Female
KW - Glucose Clamp Technique
KW - Glucose Transporter Type 4
KW - Humans
KW - Insulin
KW - Insulin, Regular, Pork
KW - Kinetics
KW - Male
KW - Middle Aged
KW - Monosaccharide Transport Proteins
KW - Muscle Proteins
KW - Muscles
KW - Rats
KW - Rats, Wistar
KW - Reference Values
KW - Time Factors
M3 - Journal article
C2 - 8279544
VL - 265
SP - E889-97
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 6 Pt 1
ER -
ID: 150332835