TY - JOUR

T1 - GLUT-4 content in plasma membrane of muscle from patients with non-insulin-dependent diabetes mellitus

AU - Lund, S

AU - Vestergaard, H

AU - Andersen, P H

AU - Schmitz, O

AU - Gøtzsche, L B

AU - Pedersen, O

PY - 1993/12

Y1 - 1993/12

N2 - The abundance of GLUT-4 protein in both total crude membrane and plasma membrane fractions of vastus lateralis muscle from 13 obese non-insulin-dependent diabetes mellitus (NIDDM) patients and 14 healthy subjects were examined in the fasting state and after supraphysiological hyperinsulinemia. In the basal state the immunoreactive mass of GLUT-4 protein both in the crude membrane preparation and in the plasma membrane fraction was similar in NIDDM patients and control subjects. Moreover, in vivo insulin exposure neither for 30 min nor for 4 h had any impact on the content of GLUT-4 protein in plasma membranes. With the use of the same methodology, antibody, and achieving the same degree of plasma membrane purification and recovery, we found, however, that intraperitoneal administration of insulin to 7-wk-old rats within 30 min increased the content of GLUT-4 protein more than twofold (P < 0.01) in the plasma membrane from red gastrocnemius and soleus muscle. In conclusion, when the subcellular fractionation method was applied to human muscle biopsies taken in the basal state, no difference could be found in the plasma membrane content of immunoreactive GLUT-4 protein between NIDDM patients and normal subjects. With this technique, we were unable to show evidence for a regulatory effect of insulin on the plasma membrane level of GLUT-4 protein in human muscle.

AB - The abundance of GLUT-4 protein in both total crude membrane and plasma membrane fractions of vastus lateralis muscle from 13 obese non-insulin-dependent diabetes mellitus (NIDDM) patients and 14 healthy subjects were examined in the fasting state and after supraphysiological hyperinsulinemia. In the basal state the immunoreactive mass of GLUT-4 protein both in the crude membrane preparation and in the plasma membrane fraction was similar in NIDDM patients and control subjects. Moreover, in vivo insulin exposure neither for 30 min nor for 4 h had any impact on the content of GLUT-4 protein in plasma membranes. With the use of the same methodology, antibody, and achieving the same degree of plasma membrane purification and recovery, we found, however, that intraperitoneal administration of insulin to 7-wk-old rats within 30 min increased the content of GLUT-4 protein more than twofold (P < 0.01) in the plasma membrane from red gastrocnemius and soleus muscle. In conclusion, when the subcellular fractionation method was applied to human muscle biopsies taken in the basal state, no difference could be found in the plasma membrane content of immunoreactive GLUT-4 protein between NIDDM patients and normal subjects. With this technique, we were unable to show evidence for a regulatory effect of insulin on the plasma membrane level of GLUT-4 protein in human muscle.

KW - 4-Nitrophenylphosphatase

KW - Animals

KW - Blood Glucose

KW - Blotting, Western

KW - C-Peptide

KW - Cell Membrane

KW - Diabetes Mellitus, Type 2

KW - Fatty Acids, Nonesterified

KW - Female

KW - Glucose Clamp Technique

KW - Glucose Transporter Type 4

KW - Humans

KW - Insulin

KW - Insulin, Regular, Pork

KW - Kinetics

KW - Male

KW - Middle Aged

KW - Monosaccharide Transport Proteins

KW - Muscle Proteins

KW - Muscles

KW - Rats

KW - Rats, Wistar

KW - Reference Values

KW - Time Factors

M3 - Journal article

C2 - 8279544

VL - 265

SP - E889-97

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 6 Pt 1

ER -