Genetic glycoengineering in mammalian cells
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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Genetic glycoengineering in mammalian cells. / Narimatsu, Yoshiki; Büll, Christian; Chen, Yen Hsi; Wandall, Hans H.; Yang, Zhang; Clausen, Henrik.
I: Journal of Biological Chemistry, Bind 296, 100448, 2021.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - Genetic glycoengineering in mammalian cells
AU - Narimatsu, Yoshiki
AU - Büll, Christian
AU - Chen, Yen Hsi
AU - Wandall, Hans H.
AU - Yang, Zhang
AU - Clausen, Henrik
PY - 2021
Y1 - 2021
N2 - Advances in nuclease-based gene-editing technologies have enabled precise, stable, and systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a plethora of opportunities for studying the glycome and exploiting glycans in biomedicine. Glycoengineering using chemical, enzymatic, and genetic approaches has a long history, and precise gene editing provides a nearly unlimited playground for stable engineering of glycosylation in mammalian cells to explore and dissect the glycome and its many biological functions. Genetic engineering of glycosylation in cells also brings studies of the glycome to the single cell level and opens up wider use and integration of data in traditional omics workflows in cell biology. The last few years have seen new applications of glycoengineering in mammalian cells with perspectives for wider use in basic and applied glycosciences, and these have already led to discoveries of functions of glycans and improved designs of glycoprotein therapeutics. Here, we review the current state of the art of genetic glycoengineering in mammalian cells and highlight emerging opportunities.
AB - Advances in nuclease-based gene-editing technologies have enabled precise, stable, and systematic genetic engineering of glycosylation capacities in mammalian cells, opening up a plethora of opportunities for studying the glycome and exploiting glycans in biomedicine. Glycoengineering using chemical, enzymatic, and genetic approaches has a long history, and precise gene editing provides a nearly unlimited playground for stable engineering of glycosylation in mammalian cells to explore and dissect the glycome and its many biological functions. Genetic engineering of glycosylation in cells also brings studies of the glycome to the single cell level and opens up wider use and integration of data in traditional omics workflows in cell biology. The last few years have seen new applications of glycoengineering in mammalian cells with perspectives for wider use in basic and applied glycosciences, and these have already led to discoveries of functions of glycans and improved designs of glycoprotein therapeutics. Here, we review the current state of the art of genetic glycoengineering in mammalian cells and highlight emerging opportunities.
U2 - 10.1016/j.jbc.2021.100448
DO - 10.1016/j.jbc.2021.100448
M3 - Review
C2 - 33617880
AN - SCOPUS:85103669738
VL - 296
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
M1 - 100448
ER -
ID: 259879499