Fragmentation of Human Neutrophil α-Defensin 4 to Combat Multidrug Resistant Bacteria
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- Fragmentation of Human Neutrophil α-Defensin 4 to Combat Multidrug Resistant Bacteria
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The occurrence and spread of multidrug-resistant bacteria is a prominent health concern. To curb this urgent threat, new innovative strategies pursuing novel antimicrobial agents are of the utmost importance. Here, we unleashed the antimicrobial activity of human neutrophil peptide-4 (HNP-4) by tryptic digestion. We identified a single 11 amino acid long fragment (HNP-41–11) with remarkable antimicrobial potential, exceeding that of the full length peptide on both mass and molar levels. Importantly, HNP-41–11 was equally bactericidal against multidrug-resistant and non-resistant strains; a potency that was further enhanced by N- and C-terminus modifications (acetylation and amidation, respectively). These observations, combined with negligible cytotoxicity not exceeding that of the full length peptide, presents proteolytic digestion of innate host-defense-peptides as a novel strategy to overcome the current health crisis related to antibiotic-resistant bacteria.
Originalsprog | Engelsk |
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Artikelnummer | 1147 |
Tidsskrift | Frontiers in Microbiology |
Vol/bind | 11 |
Antal sider | 10 |
ISSN | 1664-302X |
DOI | |
Status | Udgivet - 2020 |
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