FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule

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Standard

FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule. / Thomsen, Line Nymann; Thomsen, Preben Dybdahl; Downing, Alison; Talbot, Richard; Berg, Lise Charlotte.

I: B M C Veterinary Research, Bind 13, 106, 04.2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Thomsen, LN, Thomsen, PD, Downing, A, Talbot, R & Berg, LC 2017, 'FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule', B M C Veterinary Research, bind 13, 106. https://doi.org/10.1186/s12917-017-1003-x

APA

Thomsen, L. N., Thomsen, P. D., Downing, A., Talbot, R., & Berg, L. C. (2017). FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule. B M C Veterinary Research, 13, [106]. https://doi.org/10.1186/s12917-017-1003-x

Vancouver

Thomsen LN, Thomsen PD, Downing A, Talbot R, Berg LC. FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule. B M C Veterinary Research. 2017 apr.;13. 106. https://doi.org/10.1186/s12917-017-1003-x

Author

Thomsen, Line Nymann ; Thomsen, Preben Dybdahl ; Downing, Alison ; Talbot, Richard ; Berg, Lise Charlotte. / FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule. I: B M C Veterinary Research. 2017 ; Bind 13.

Bibtex

@article{8cadc03b7ff241fca996a43a97ff49a6,
title = "FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule",
abstract = "BackgroundThe synovial membrane lines the luminal side of the joint capsule in synovial joints. It maintains joint homeostasis and plays a crucial role in equine joint pathology. When trauma or inflammation is induced in a joint, the synovial membrane influences progression of joint damage. Equine synovial membrane research is hampered by a lack of markers of fibroblast-like synoviocytes (FLS) to distinguish FLS from other fibroblast-like cells in musculoskeletal connective tissues. The aim of this study is to identify potential FLS markers of the equine synovial membrane using microarray to compare between gene expression in equine synovial membrane and the joint capsule in metacarpophalangeal joints.ResultsMicroarray analysis of tissues from 6 horses resulted in 1167 up-regulated genes in synovial membrane compared with joint capsule. Pathway analysis resulted in 241 candidate genes. Of these, 15 genes were selected for further confirmation as genes potentially expressed by fibroblast-like synoviocytes. Four genes: FOXO1, PXK, PYCARD and SAMD9L were confirmed in 9 horses by qPCR as differentially expressed in synovial membrane compared to joint capsule.ConclusionsIn conclusion, FOXO1, PXK, PYCARD and SAMD9L were confirmed as differentially expressed in synovial membrane compared to joint capsule. These four genes are potential markers of fibroblast-like synoviocytes of the synovial membrane. As these genes are overexpressed in synovial membrane compared to joint capsule, these genes could shed light on synovial membrane physiology and its role in joint disease.",
keywords = "Synovial membrane, Fibroblast-like synoviocytes, FOXO1, PXK, PYCARD, SAMD9L, Equine, Marker",
author = "Thomsen, {Line Nymann} and Thomsen, {Preben Dybdahl} and Alison Downing and Richard Talbot and Berg, {Lise Charlotte}",
year = "2017",
month = apr,
doi = "10.1186/s12917-017-1003-x",
language = "English",
volume = "13",
journal = "B M C Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule

AU - Thomsen, Line Nymann

AU - Thomsen, Preben Dybdahl

AU - Downing, Alison

AU - Talbot, Richard

AU - Berg, Lise Charlotte

PY - 2017/4

Y1 - 2017/4

N2 - BackgroundThe synovial membrane lines the luminal side of the joint capsule in synovial joints. It maintains joint homeostasis and plays a crucial role in equine joint pathology. When trauma or inflammation is induced in a joint, the synovial membrane influences progression of joint damage. Equine synovial membrane research is hampered by a lack of markers of fibroblast-like synoviocytes (FLS) to distinguish FLS from other fibroblast-like cells in musculoskeletal connective tissues. The aim of this study is to identify potential FLS markers of the equine synovial membrane using microarray to compare between gene expression in equine synovial membrane and the joint capsule in metacarpophalangeal joints.ResultsMicroarray analysis of tissues from 6 horses resulted in 1167 up-regulated genes in synovial membrane compared with joint capsule. Pathway analysis resulted in 241 candidate genes. Of these, 15 genes were selected for further confirmation as genes potentially expressed by fibroblast-like synoviocytes. Four genes: FOXO1, PXK, PYCARD and SAMD9L were confirmed in 9 horses by qPCR as differentially expressed in synovial membrane compared to joint capsule.ConclusionsIn conclusion, FOXO1, PXK, PYCARD and SAMD9L were confirmed as differentially expressed in synovial membrane compared to joint capsule. These four genes are potential markers of fibroblast-like synoviocytes of the synovial membrane. As these genes are overexpressed in synovial membrane compared to joint capsule, these genes could shed light on synovial membrane physiology and its role in joint disease.

AB - BackgroundThe synovial membrane lines the luminal side of the joint capsule in synovial joints. It maintains joint homeostasis and plays a crucial role in equine joint pathology. When trauma or inflammation is induced in a joint, the synovial membrane influences progression of joint damage. Equine synovial membrane research is hampered by a lack of markers of fibroblast-like synoviocytes (FLS) to distinguish FLS from other fibroblast-like cells in musculoskeletal connective tissues. The aim of this study is to identify potential FLS markers of the equine synovial membrane using microarray to compare between gene expression in equine synovial membrane and the joint capsule in metacarpophalangeal joints.ResultsMicroarray analysis of tissues from 6 horses resulted in 1167 up-regulated genes in synovial membrane compared with joint capsule. Pathway analysis resulted in 241 candidate genes. Of these, 15 genes were selected for further confirmation as genes potentially expressed by fibroblast-like synoviocytes. Four genes: FOXO1, PXK, PYCARD and SAMD9L were confirmed in 9 horses by qPCR as differentially expressed in synovial membrane compared to joint capsule.ConclusionsIn conclusion, FOXO1, PXK, PYCARD and SAMD9L were confirmed as differentially expressed in synovial membrane compared to joint capsule. These four genes are potential markers of fibroblast-like synoviocytes of the synovial membrane. As these genes are overexpressed in synovial membrane compared to joint capsule, these genes could shed light on synovial membrane physiology and its role in joint disease.

KW - Synovial membrane

KW - Fibroblast-like synoviocytes

KW - FOXO1

KW - PXK

KW - PYCARD

KW - SAMD9L

KW - Equine

KW - Marker

U2 - 10.1186/s12917-017-1003-x

DO - 10.1186/s12917-017-1003-x

M3 - Journal article

C2 - 28410619

VL - 13

JO - B M C Veterinary Research

JF - B M C Veterinary Research

SN - 1746-6148

M1 - 106

ER -

ID: 186319210