Formation and structure of fluorescent silver nanoclusters at interfacial binding sites facilitating oligomerization of DNA hairpins
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Formation and structure of fluorescent silver nanoclusters at interfacial binding sites facilitating oligomerization of DNA hairpins. / Geczy, Reka; Christensen, Niels Johan; Rasmussen, Kim K; Kálomista, Ildikó; Tiwari, Manish K; Shah, Pratik; Yang, Seong Wook; Bjerrum, Morten J; Thulstrup, Peter Waaben.
I: Angewandte Chemie, Int. Ed., Bind 59, Nr. 37, 2020, s. 16091-16097.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Formation and structure of fluorescent silver nanoclusters at interfacial binding sites facilitating oligomerization of DNA hairpins
AU - Geczy, Reka
AU - Christensen, Niels Johan
AU - Rasmussen, Kim K
AU - Kálomista, Ildikó
AU - Tiwari, Manish K
AU - Shah, Pratik
AU - Yang, Seong Wook
AU - Bjerrum, Morten J
AU - Thulstrup, Peter Waaben
N1 - © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2020
Y1 - 2020
N2 - Fluorescent, DNA-stabilized silver nanoclusters (DNA-AgNCs) are applied in a range of applications within nanoscience and nanotechnology. However, their diverse optical properties, mechanism of formation, and aspects of their composition remain unexplored, making the rational design of nanocluster probes challenging. Here, a synthetic procedure is described for obtaining a high yield of emissive DNA-AgNCs with a C-loop hairpin DNA sequence, with subsequent purification by size-exclusion chromatography (SEC). Through a combination of optical spectroscopy, gel electrophoresis, ICP-MS, and small-angle X-ray scattering (SAXS) in conjunction with the systematic study of various DNA sequences, the low-resolution structure and mechanism of the formation of AgNCs were investigated. Data indicate that fluorescent DNA-AgNCs self-assemble via a head-to-head binding of two DNA hairpins, bridged by a silver nanocluster, resulting in the modelling of a dimeric structure harboring an Ag 12 cluster.
AB - Fluorescent, DNA-stabilized silver nanoclusters (DNA-AgNCs) are applied in a range of applications within nanoscience and nanotechnology. However, their diverse optical properties, mechanism of formation, and aspects of their composition remain unexplored, making the rational design of nanocluster probes challenging. Here, a synthetic procedure is described for obtaining a high yield of emissive DNA-AgNCs with a C-loop hairpin DNA sequence, with subsequent purification by size-exclusion chromatography (SEC). Through a combination of optical spectroscopy, gel electrophoresis, ICP-MS, and small-angle X-ray scattering (SAXS) in conjunction with the systematic study of various DNA sequences, the low-resolution structure and mechanism of the formation of AgNCs were investigated. Data indicate that fluorescent DNA-AgNCs self-assemble via a head-to-head binding of two DNA hairpins, bridged by a silver nanocluster, resulting in the modelling of a dimeric structure harboring an Ag 12 cluster.
U2 - 10.1002/anie.202005102
DO - 10.1002/anie.202005102
M3 - Journal article
C2 - 32516466
VL - 59
SP - 16091
EP - 16097
JO - Angewandte Chemie International Edition
JF - Angewandte Chemie International Edition
SN - 1433-7851
IS - 37
ER -
ID: 243343590