Expression of the Otx2 homeobox gene in the developing mammalian brain: embryonic and adult expression in the pineal gland
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Expression of the Otx2 homeobox gene in the developing mammalian brain: embryonic and adult expression in the pineal gland. / Rath, Martin F; Muñoz, Estela; Ganguly, Surajit; Morin, Fabrice; Shi, Qiong; Klein, David C; Møller, Morten.
I: Journal of Neurochemistry, Bind 97, Nr. 2, 2006, s. 556-566.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Expression of the Otx2 homeobox gene in the developing mammalian brain: embryonic and adult expression in the pineal gland
AU - Rath, Martin F
AU - Muñoz, Estela
AU - Ganguly, Surajit
AU - Morin, Fabrice
AU - Shi, Qiong
AU - Klein, David C
AU - Møller, Morten
PY - 2006
Y1 - 2006
N2 - Otx2 is a vertebrate homeobox gene, which has been found to be essential for the development of rostral brain regions and appears to play a role in the development of retinal photoreceptor cells and pinealocytes. In this study, the temporal expression pattern of Otx2 was revealed in the rat brain, with special emphasis on the pineal gland throughout late embryonic and postnatal stages. Widespread high expression of Otx2 in the embryonic brain becomes progressively restricted in the adult to the pineal gland. Crx (cone-rod homeobox), a downstream target gene of Otx2, showed a pineal expression pattern similar to that of Otx2, although there was a distinct lag in time of onset. Otx2 protein was identified in pineal extracts and found to be localized in pinealocytes. Total pineal Otx2 mRNA did not show day-night variation, nor was it influenced by removal of the sympathetic input, indicating that the level of Otx2 mRNA appears to be independent of the photoneural input to the gland. Our results are consistent with the view that pineal expression of Otx2 is required for development and we hypothesize that it plays a role in the adult in controlling the expression of the cluster of genes associated with phototransduction and melatonin synthesis.
AB - Otx2 is a vertebrate homeobox gene, which has been found to be essential for the development of rostral brain regions and appears to play a role in the development of retinal photoreceptor cells and pinealocytes. In this study, the temporal expression pattern of Otx2 was revealed in the rat brain, with special emphasis on the pineal gland throughout late embryonic and postnatal stages. Widespread high expression of Otx2 in the embryonic brain becomes progressively restricted in the adult to the pineal gland. Crx (cone-rod homeobox), a downstream target gene of Otx2, showed a pineal expression pattern similar to that of Otx2, although there was a distinct lag in time of onset. Otx2 protein was identified in pineal extracts and found to be localized in pinealocytes. Total pineal Otx2 mRNA did not show day-night variation, nor was it influenced by removal of the sympathetic input, indicating that the level of Otx2 mRNA appears to be independent of the photoneural input to the gland. Our results are consistent with the view that pineal expression of Otx2 is required for development and we hypothesize that it plays a role in the adult in controlling the expression of the cluster of genes associated with phototransduction and melatonin synthesis.
KW - Age Factors
KW - Animals
KW - Animals, Newborn
KW - Blotting, Northern
KW - Blotting, Western
KW - Brain
KW - Cloning, Molecular
KW - Embryo, Mammalian
KW - Gene Expression Regulation, Developmental
KW - Immunohistochemistry
KW - Otx Transcription Factors
KW - Pineal Gland
KW - Rats
KW - Sequence Analysis, DNA
U2 - 10.1111/j.1471-4159.2006.03773.x
DO - 10.1111/j.1471-4159.2006.03773.x
M3 - Journal article
C2 - 16539656
VL - 97
SP - 556
EP - 566
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 2
ER -
ID: 1092288