Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus. / Hansen, Torben; Bjørbaek, C; Vestergaard, H; Grønskov, K; Bak, J F; Pedersen, O.

I: The Journal of clinical endocrinology and metabolism, Bind 77, Nr. 6, 12.1993, s. 1500-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hansen, T, Bjørbaek, C, Vestergaard, H, Grønskov, K, Bak, JF & Pedersen, O 1993, 'Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus', The Journal of clinical endocrinology and metabolism, bind 77, nr. 6, s. 1500-5.

APA

Hansen, T., Bjørbaek, C., Vestergaard, H., Grønskov, K., Bak, J. F., & Pedersen, O. (1993). Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus. The Journal of clinical endocrinology and metabolism, 77(6), 1500-5.

Vancouver

Hansen T, Bjørbaek C, Vestergaard H, Grønskov K, Bak JF, Pedersen O. Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus. The Journal of clinical endocrinology and metabolism. 1993 dec.;77(6):1500-5.

Author

Hansen, Torben ; Bjørbaek, C ; Vestergaard, H ; Grønskov, K ; Bak, J F ; Pedersen, O. / Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus. I: The Journal of clinical endocrinology and metabolism. 1993 ; Bind 77, Nr. 6. s. 1500-5.

Bibtex

@article{a01c4531c2334332941b968500c793e8,
title = "Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus",
abstract = "Due to alternative splicing of exon 11 of the receptor gene, the human insulin receptor exists in two forms, that have distinct tissue-specific expression and are functionally different. Needle biopsies obtained from vastus lateralis muscle from 20 patients with noninsulin-dependent diabetes mellitus (NIDDM) and 20 normal control subjects were analyzed for the relative expression of insulin receptor mRNA variants in a novel assay using fluorescence-labeled primers and subsequent analysis on an automated DNA sequencer. In subgroups of patients and control subjects, insulin binding and tyrosine kinase activity were examined in wheat germ agglutinin-purified insulin receptors isolated from muscle biopsies. Moreover, insulin-stimulated glucose disposal was studied by means of the euglycemic hyperinsulinemic clamp technique. No difference in the relative expression of spliced variants of the insulin receptor mRNA was observed (control subjects, 71.4 +/- 1.3% insulin receptor mRNA with exon 11; NIDDM patients, 71.5 +/- 1.3% insulin receptor mRNA with exon 11). No significant interrelationships were demonstrated among the relative expression of insulin receptor mRNA variants, insulin binding, and tyrosine kinase activity toward the exogenous substrate poly(Glu-Tyr(4:1)). Furthermore, no significant relationship was demonstrated between the glucose disposal rate and the relative expression of insulin receptor splice variants. In conclusion, in skeletal muscle from both normal control subjects and NIDDM patients, the proportion of insulin receptor mRNA with exon 11 is about 70%. In addition, no significant correlations exist among insulin binding, insulin receptor tyrosine kinase activity, glucose disposal rate, and expression of alternative spliced insulin receptors in human skeletal muscle.",
keywords = "Adult, Aged, Alternative Splicing, Diabetes Mellitus, Type 2, Female, Humans, Male, Middle Aged, Muscles, Polymerase Chain Reaction, RNA, Messenger, Receptor, Insulin",
author = "Torben Hansen and C Bj{\o}rbaek and H Vestergaard and K Gr{\o}nskov and Bak, {J F} and O Pedersen",
year = "1993",
month = dec,
language = "English",
volume = "77",
pages = "1500--5",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Expression of insulin receptor spliced variants and their functional correlates in muscle from patients with non-insulin-dependent diabetes mellitus

AU - Hansen, Torben

AU - Bjørbaek, C

AU - Vestergaard, H

AU - Grønskov, K

AU - Bak, J F

AU - Pedersen, O

PY - 1993/12

Y1 - 1993/12

N2 - Due to alternative splicing of exon 11 of the receptor gene, the human insulin receptor exists in two forms, that have distinct tissue-specific expression and are functionally different. Needle biopsies obtained from vastus lateralis muscle from 20 patients with noninsulin-dependent diabetes mellitus (NIDDM) and 20 normal control subjects were analyzed for the relative expression of insulin receptor mRNA variants in a novel assay using fluorescence-labeled primers and subsequent analysis on an automated DNA sequencer. In subgroups of patients and control subjects, insulin binding and tyrosine kinase activity were examined in wheat germ agglutinin-purified insulin receptors isolated from muscle biopsies. Moreover, insulin-stimulated glucose disposal was studied by means of the euglycemic hyperinsulinemic clamp technique. No difference in the relative expression of spliced variants of the insulin receptor mRNA was observed (control subjects, 71.4 +/- 1.3% insulin receptor mRNA with exon 11; NIDDM patients, 71.5 +/- 1.3% insulin receptor mRNA with exon 11). No significant interrelationships were demonstrated among the relative expression of insulin receptor mRNA variants, insulin binding, and tyrosine kinase activity toward the exogenous substrate poly(Glu-Tyr(4:1)). Furthermore, no significant relationship was demonstrated between the glucose disposal rate and the relative expression of insulin receptor splice variants. In conclusion, in skeletal muscle from both normal control subjects and NIDDM patients, the proportion of insulin receptor mRNA with exon 11 is about 70%. In addition, no significant correlations exist among insulin binding, insulin receptor tyrosine kinase activity, glucose disposal rate, and expression of alternative spliced insulin receptors in human skeletal muscle.

AB - Due to alternative splicing of exon 11 of the receptor gene, the human insulin receptor exists in two forms, that have distinct tissue-specific expression and are functionally different. Needle biopsies obtained from vastus lateralis muscle from 20 patients with noninsulin-dependent diabetes mellitus (NIDDM) and 20 normal control subjects were analyzed for the relative expression of insulin receptor mRNA variants in a novel assay using fluorescence-labeled primers and subsequent analysis on an automated DNA sequencer. In subgroups of patients and control subjects, insulin binding and tyrosine kinase activity were examined in wheat germ agglutinin-purified insulin receptors isolated from muscle biopsies. Moreover, insulin-stimulated glucose disposal was studied by means of the euglycemic hyperinsulinemic clamp technique. No difference in the relative expression of spliced variants of the insulin receptor mRNA was observed (control subjects, 71.4 +/- 1.3% insulin receptor mRNA with exon 11; NIDDM patients, 71.5 +/- 1.3% insulin receptor mRNA with exon 11). No significant interrelationships were demonstrated among the relative expression of insulin receptor mRNA variants, insulin binding, and tyrosine kinase activity toward the exogenous substrate poly(Glu-Tyr(4:1)). Furthermore, no significant relationship was demonstrated between the glucose disposal rate and the relative expression of insulin receptor splice variants. In conclusion, in skeletal muscle from both normal control subjects and NIDDM patients, the proportion of insulin receptor mRNA with exon 11 is about 70%. In addition, no significant correlations exist among insulin binding, insulin receptor tyrosine kinase activity, glucose disposal rate, and expression of alternative spliced insulin receptors in human skeletal muscle.

KW - Adult

KW - Aged

KW - Alternative Splicing

KW - Diabetes Mellitus, Type 2

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Muscles

KW - Polymerase Chain Reaction

KW - RNA, Messenger

KW - Receptor, Insulin

M3 - Journal article

C2 - 8263133

VL - 77

SP - 1500

EP - 1505

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 6

ER -

ID: 92194060