Evaluation of the in vitro effects of local anesthetics on equine chondrocytes and fibroblast-like synoviocytes

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OBJECTIVE To investigate the in vitro effects of clinically relevant concentrations of the local anesthetics (LAs) bupivacaine, lidocaine, lidocaine with preservative (LP), mepivacaine, and ropivacaine on equine chondrocyte and fibroblast-like synoviocyte (FLS) viability. SAMPLES Chondrocytes and FLSs of the metacarpophalangeal joints of 4 healthy adult horses. PROCEDURES Viability of chondrocytes and FLSs was determined with 3 assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate de-hydrogenase (LDH), and trypan blue (TB) exclusion (only FLS). Viability was assessed after 30-and 60-minute exposures to 0.0625%, 0.125%, and 0.25% bupivacaine; 0.25%, 0.5%, and 1% lidocaine; 0.25%, 0.5%, and 1% LP; 0.25%, 0.5%, and 1% mepivacaine; and 0.125%, 0.25%, and 0.5% ropivacaine. RESULTS Viability of chondrocytes was significantly decreased with exposure to 0.25% bupivacaine, 1% lidocaine, 1% LP, 1% mepivacaine, and 0.25% ropiva-caine. Viability of FLSs was significantly decreased with exposure to 0.25% bupivacaine, 1% mepivacaine, 1% LP, and 0.5% ropivacaine. CONCLUSIONS AND CLINICAL RELEVANCE Clinically relevant concentrations of LAs had in vitro time-and concentra-tion-dependent cytotoxicity for chondrocytes and FLSs isolated from the metacarpophalangeal joints of healthy horses. Bupivacaine was more toxic to chondrocytes than lidocaine, mepivacaine, and ropivacaine, whereas bu-pivacaine, LP, mepivacaine, and ropivacaine were more toxic to FLSs than preservative-free lidocaine. Several LAs may negatively affect chondrocyte and FLS viability. (Am J Vet Res 2021;82:478–486).

TidsskriftAmerican Journal of Veterinary Research
Udgave nummer6
Sider (fra-til)478-486
Antal sider9
StatusUdgivet - 2021

Bibliografisk note

Funding Information:
Funded in part by Boehringer Ingelheim Denmark A/S. Con-sumables were provided by E-vet A/S. None of the authors have any financial or personal relation-ships that could inappropriately influence or bias the content of the article. The authors acknowledge horse owners that permitted their horses for inclusion in the study.

Publisher Copyright:
© 2021, American Veterinary Medical Association. All rights reserved.

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