Error-tolerant identification of peptides in sequence databases by peptide sequence tags

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Error-tolerant identification of peptides in sequence databases by peptide sequence tags. / Mann, M; Wilm, M.

I: Analytical Chemistry, Bind 66, Nr. 24, 15.12.1994, s. 4390-9.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mann, M & Wilm, M 1994, 'Error-tolerant identification of peptides in sequence databases by peptide sequence tags', Analytical Chemistry, bind 66, nr. 24, s. 4390-9. https://doi.org/10.1021/ac00096a002

APA

Mann, M., & Wilm, M. (1994). Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Analytical Chemistry, 66(24), 4390-9. https://doi.org/10.1021/ac00096a002

Vancouver

Mann M, Wilm M. Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Analytical Chemistry. 1994 dec. 15;66(24):4390-9. https://doi.org/10.1021/ac00096a002

Author

Mann, M ; Wilm, M. / Error-tolerant identification of peptides in sequence databases by peptide sequence tags. I: Analytical Chemistry. 1994 ; Bind 66, Nr. 24. s. 4390-9.

Bibtex

@article{9a79c9db75f24f278b180cf9f4dda57a,
title = "Error-tolerant identification of peptides in sequence databases by peptide sequence tags",
abstract = "We demonstrate a new approach to the identification of mass spectrometrically fragmented peptides. A fragmentation spectrum usually contains a short, easily identifiable series of sequence ions, which yields a partial sequence. This partial sequence divides the peptide into three parts-regions 1, 2, and 3-characterized by the added mass m1 of region 1, the partial sequence of region 2, and the added mass m3 of region 3. We call the construct, m1 partial sequence m3, a {"}peptide sequence tag{"} and show that it is a highly specific identifier of the peptide. An algorithm developed here that uses the sequence tag to find the peptide in a sequence database is up to 1 million-fold more discriminating than the partial sequence information alone. Peptides can be identified even in the presence of an unknown posttranslational modification or an amino acid substitution between an entry in the sequence database and the measured peptide. These concepts are demonstrated with model and practical examples of electrospray mass spectrometry/mass spectrometry of tryptic peptides. Just two to three amino acid residues derived by fragmentation are enough to identify these peptides. In peptide mapping applications, even less information is necessary.",
keywords = "Algorithms, Amino Acid Sequence, Animals, Chickens, Databases, Factual, Mass Spectrometry, Molecular Sequence Data, Muramidase/chemistry, Peptide Mapping, Peptides/analysis, Software",
author = "M Mann and M Wilm",
year = "1994",
month = dec,
day = "15",
doi = "10.1021/ac00096a002",
language = "English",
volume = "66",
pages = "4390--9",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "24",

}

RIS

TY - JOUR

T1 - Error-tolerant identification of peptides in sequence databases by peptide sequence tags

AU - Mann, M

AU - Wilm, M

PY - 1994/12/15

Y1 - 1994/12/15

N2 - We demonstrate a new approach to the identification of mass spectrometrically fragmented peptides. A fragmentation spectrum usually contains a short, easily identifiable series of sequence ions, which yields a partial sequence. This partial sequence divides the peptide into three parts-regions 1, 2, and 3-characterized by the added mass m1 of region 1, the partial sequence of region 2, and the added mass m3 of region 3. We call the construct, m1 partial sequence m3, a "peptide sequence tag" and show that it is a highly specific identifier of the peptide. An algorithm developed here that uses the sequence tag to find the peptide in a sequence database is up to 1 million-fold more discriminating than the partial sequence information alone. Peptides can be identified even in the presence of an unknown posttranslational modification or an amino acid substitution between an entry in the sequence database and the measured peptide. These concepts are demonstrated with model and practical examples of electrospray mass spectrometry/mass spectrometry of tryptic peptides. Just two to three amino acid residues derived by fragmentation are enough to identify these peptides. In peptide mapping applications, even less information is necessary.

AB - We demonstrate a new approach to the identification of mass spectrometrically fragmented peptides. A fragmentation spectrum usually contains a short, easily identifiable series of sequence ions, which yields a partial sequence. This partial sequence divides the peptide into three parts-regions 1, 2, and 3-characterized by the added mass m1 of region 1, the partial sequence of region 2, and the added mass m3 of region 3. We call the construct, m1 partial sequence m3, a "peptide sequence tag" and show that it is a highly specific identifier of the peptide. An algorithm developed here that uses the sequence tag to find the peptide in a sequence database is up to 1 million-fold more discriminating than the partial sequence information alone. Peptides can be identified even in the presence of an unknown posttranslational modification or an amino acid substitution between an entry in the sequence database and the measured peptide. These concepts are demonstrated with model and practical examples of electrospray mass spectrometry/mass spectrometry of tryptic peptides. Just two to three amino acid residues derived by fragmentation are enough to identify these peptides. In peptide mapping applications, even less information is necessary.

KW - Algorithms

KW - Amino Acid Sequence

KW - Animals

KW - Chickens

KW - Databases, Factual

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Muramidase/chemistry

KW - Peptide Mapping

KW - Peptides/analysis

KW - Software

U2 - 10.1021/ac00096a002

DO - 10.1021/ac00096a002

M3 - Journal article

C2 - 7847635

VL - 66

SP - 4390

EP - 4399

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 24

ER -

ID: 246724399