Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency

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Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. / Martin Gonzalez, Javier; Morgani, Sophie M; Bone, Robert A; Bonderup, Kasper; Abelechian, Sahar; Brakebusch, Cord; Brickman, Joshua M.

I: Stem Cell Reports, Bind 7, Nr. 2, 09.08.2016, s. 177-91.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Martin Gonzalez, J, Morgani, SM, Bone, RA, Bonderup, K, Abelechian, S, Brakebusch, C & Brickman, JM 2016, 'Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency', Stem Cell Reports, bind 7, nr. 2, s. 177-91. https://doi.org/10.1016/j.stemcr.2016.07.009

APA

Martin Gonzalez, J., Morgani, S. M., Bone, R. A., Bonderup, K., Abelechian, S., Brakebusch, C., & Brickman, J. M. (2016). Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports, 7(2), 177-91. https://doi.org/10.1016/j.stemcr.2016.07.009

Vancouver

Martin Gonzalez J, Morgani SM, Bone RA, Bonderup K, Abelechian S, Brakebusch C o.a. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016 aug. 9;7(2):177-91. https://doi.org/10.1016/j.stemcr.2016.07.009

Author

Martin Gonzalez, Javier ; Morgani, Sophie M ; Bone, Robert A ; Bonderup, Kasper ; Abelechian, Sahar ; Brakebusch, Cord ; Brickman, Joshua M. / Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. I: Stem Cell Reports. 2016 ; Bind 7, Nr. 2. s. 177-91.

Bibtex

@article{d64acd9d4c3740e7a5913e331a683fb4,

title = "Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency",

abstract = "Embryonic stem cells (ESCs) are cell lines derived from the mammalian pre-implantation embryo. Here we assess the impact of derivation and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultured in these conditions showed a transcriptional correlation with early pre-implantation embryos (E1.5-E3.5) and contributed to development from the 2-cell stage. Conversely, the transcriptome of serum-cultured ESCs correlated with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to another developmental state merely by adaptation to another culture condition.",

keywords = "Journal Article",

author = "{Martin Gonzalez}, Javier and Morgani, {Sophie M} and Bone, {Robert A} and Kasper Bonderup and Sahar Abelechian and Cord Brakebusch and Brickman, {Joshua M}",

year = "2016",

month = aug,

day = "9",

doi = "10.1016/j.stemcr.2016.07.009",

language = "English",

volume = "7",

pages = "177--91",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency

AU - Martin Gonzalez, Javier

AU - Morgani, Sophie M

AU - Bone, Robert A

AU - Bonderup, Kasper

AU - Abelechian, Sahar

AU - Brakebusch, Cord

AU - Brickman, Joshua M

PY - 2016/8/9

Y1 - 2016/8/9

N2 - Embryonic stem cells (ESCs) are cell lines derived from the mammalian pre-implantation embryo. Here we assess the impact of derivation and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultured in these conditions showed a transcriptional correlation with early pre-implantation embryos (E1.5-E3.5) and contributed to development from the 2-cell stage. Conversely, the transcriptome of serum-cultured ESCs correlated with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to another developmental state merely by adaptation to another culture condition.

AB - Embryonic stem cells (ESCs) are cell lines derived from the mammalian pre-implantation embryo. Here we assess the impact of derivation and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultured in these conditions showed a transcriptional correlation with early pre-implantation embryos (E1.5-E3.5) and contributed to development from the 2-cell stage. Conversely, the transcriptome of serum-cultured ESCs correlated with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to another developmental state merely by adaptation to another culture condition.

KW - Journal Article

U2 - 10.1016/j.stemcr.2016.07.009

DO - 10.1016/j.stemcr.2016.07.009

M3 - Journal article

C2 - 27509134

VL - 7

SP - 177

EP - 191

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 2

ER -

ID: 167470304