Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain

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Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain. / Nazerai, Loulieta; Buus, Søren; Stryhn, Anette; Thomsen, Allan Randrup; Christensen, Jan Pravsgaard.

I: Viruses, Bind 13, Nr. 11, 2215, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nazerai, L, Buus, S, Stryhn, A, Thomsen, AR & Christensen, JP 2021, 'Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain', Viruses, bind 13, nr. 11, 2215. https://doi.org/10.3390/v13112215

APA

Nazerai, L., Buus, S., Stryhn, A., Thomsen, A. R., & Christensen, J. P. (2021). Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain. Viruses, 13(11), [2215]. https://doi.org/10.3390/v13112215

Vancouver

Nazerai L, Buus S, Stryhn A, Thomsen AR, Christensen JP. Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain. Viruses. 2021;13(11). 2215. https://doi.org/10.3390/v13112215

Author

Nazerai, Loulieta ; Buus, Søren ; Stryhn, Anette ; Thomsen, Allan Randrup ; Christensen, Jan Pravsgaard. / Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain. I: Viruses. 2021 ; Bind 13, Nr. 11.

Bibtex

@article{06f47aa3539343fb857cf48c01041a11,

title = "Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain",

abstract = "It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.",

keywords = "CD8 T cell response, MHC-II invariant chain, NS1/NS2, Vaccine, Zika virus",

author = "Loulieta Nazerai and S{\o}ren Buus and Anette Stryhn and Thomsen, {Allan Randrup} and Christensen, {Jan Pravsgaard}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

doi = "10.3390/v13112215",

language = "English",

volume = "13",

journal = "Viruses",

issn = "1999-4915",

publisher = "M D P I AG",

number = "11",

}

RIS

TY - JOUR

T1 - Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain

AU - Nazerai, Loulieta

AU - Buus, Søren

AU - Stryhn, Anette

AU - Thomsen, Allan Randrup

AU - Christensen, Jan Pravsgaard

PY - 2021

Y1 - 2021

N2 - It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.

AB - It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.

KW - CD8 T cell response

KW - MHC-II invariant chain

KW - NS1/NS2

KW - Vaccine

KW - Zika virus

U2 - 10.3390/v13112215

DO - 10.3390/v13112215

M3 - Journal article

C2 - 34835021

AN - SCOPUS:85118742247

VL - 13

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 11

M1 - 2215

ER -

ID: 285311839