Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans

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Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans. / Johansson, Pär I; Bergström, Anita; Aachmann-Andersen, Niels Jacob; Meyer, Martin A S; Ostrowski, Sisse Rye; Nordsborg, Nikolai Baastrup; Olsen, Niels Vidiendal.

I: Frontiers in Physiology, Bind 5, 459, 2014.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Johansson, PI, Bergström, A, Aachmann-Andersen, NJ, Meyer, MAS, Ostrowski, SR, Nordsborg, NB & Olsen, NV 2014, 'Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans', Frontiers in Physiology, bind 5, 459. https://doi.org/10.3389/fphys.2014.00459

APA

Johansson, P. I., Bergström, A., Aachmann-Andersen, N. J., Meyer, M. A. S., Ostrowski, S. R., Nordsborg, N. B., & Olsen, N. V. (2014). Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans. Frontiers in Physiology, 5, [459]. https://doi.org/10.3389/fphys.2014.00459

Vancouver

Johansson PI, Bergström A, Aachmann-Andersen NJ, Meyer MAS, Ostrowski SR, Nordsborg NB o.a. Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans. Frontiers in Physiology. 2014;5. 459. https://doi.org/10.3389/fphys.2014.00459

Author

Johansson, Pär I ; Bergström, Anita ; Aachmann-Andersen, Niels Jacob ; Meyer, Martin A S ; Ostrowski, Sisse Rye ; Nordsborg, Nikolai Baastrup ; Olsen, Niels Vidiendal. / Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans. I: Frontiers in Physiology. 2014 ; Bind 5.

Bibtex

@article{883c98e447774e98bd63c84e3993fe93,
title = "Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans",
abstract = "INTRODUCTION: Hypoxia is associated with increased capillary permeability. This study tested whether acute hypobaric hypoxia involves degradation of the endothelial glycocalyx.METHODS: We exposed 12 subjects to acute hypobaric hypoxia (equivalent to 4500 m for 2-4 h) and measured venous blood concentrations of biomarkers reflecting endothelial and glycocalyx degradation (catecholamines, syndecan-1, soluble CD40 ligand, protein C, soluble thrombomodulin, tissue-type plasminogen activators, histone-complexed DNA fragments, and nitrite/nitrate). Endothelial function was assessed by the hyperemic response to brachial artery occlusion by peripheral arterial tonometry.RESULTS: Compared with normoxic baseline levels, hypoxia increased concentrations of syndecan-1 from 22 (95{\%} confidence interval: 17-27) to 25 (19-30) ng/ml (p < 0.02) and protein C from 76 (70-83){\%} to 81 (74-88){\%} (p < 0.02). Nitrite/nitrate decreased from 23 (18-27) μM at baseline to 19 (14-24) μM and 18 (14-21) μM in hypoxia and recovery, respectively (p < 0.05). Other biomarkers remained unchanged. The post-occlusion/pre-occlusion ratio (reactive hyperemia index, RHI) decreased from 1.80 (1.52-2.07) in normoxia to 1.62 (1.28-1.96) after 2-4 h of hypobaric hypoxia and thereafter increased to 2.43 (1.99-2.86) during normoxic recovery (p < 0.01).CONCLUSIONS: The increase in syndecan-1 and protein C suggests that acute hypobaric hypoxia produces a minor degree of glycocalyx degradation and overall cellular damage. After hypoxia RHI rebounded to higher than baseline levels suggesting improved endothelial functionality.",
author = "Johansson, {P{\"a}r I} and Anita Bergstr{\"o}m and Aachmann-Andersen, {Niels Jacob} and Meyer, {Martin A S} and Ostrowski, {Sisse Rye} and Nordsborg, {Nikolai Baastrup} and Olsen, {Niels Vidiendal}",
note = "CURIS 2014 NEXS 378",
year = "2014",
doi = "10.3389/fphys.2014.00459",
language = "English",
volume = "5",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Effect of acute hypobaric hypoxia on the endothelial glycocalyx and digital reactive hyperemia in humans

AU - Johansson, Pär I

AU - Bergström, Anita

AU - Aachmann-Andersen, Niels Jacob

AU - Meyer, Martin A S

AU - Ostrowski, Sisse Rye

AU - Nordsborg, Nikolai Baastrup

AU - Olsen, Niels Vidiendal

N1 - CURIS 2014 NEXS 378

PY - 2014

Y1 - 2014

N2 - INTRODUCTION: Hypoxia is associated with increased capillary permeability. This study tested whether acute hypobaric hypoxia involves degradation of the endothelial glycocalyx.METHODS: We exposed 12 subjects to acute hypobaric hypoxia (equivalent to 4500 m for 2-4 h) and measured venous blood concentrations of biomarkers reflecting endothelial and glycocalyx degradation (catecholamines, syndecan-1, soluble CD40 ligand, protein C, soluble thrombomodulin, tissue-type plasminogen activators, histone-complexed DNA fragments, and nitrite/nitrate). Endothelial function was assessed by the hyperemic response to brachial artery occlusion by peripheral arterial tonometry.RESULTS: Compared with normoxic baseline levels, hypoxia increased concentrations of syndecan-1 from 22 (95% confidence interval: 17-27) to 25 (19-30) ng/ml (p < 0.02) and protein C from 76 (70-83)% to 81 (74-88)% (p < 0.02). Nitrite/nitrate decreased from 23 (18-27) μM at baseline to 19 (14-24) μM and 18 (14-21) μM in hypoxia and recovery, respectively (p < 0.05). Other biomarkers remained unchanged. The post-occlusion/pre-occlusion ratio (reactive hyperemia index, RHI) decreased from 1.80 (1.52-2.07) in normoxia to 1.62 (1.28-1.96) after 2-4 h of hypobaric hypoxia and thereafter increased to 2.43 (1.99-2.86) during normoxic recovery (p < 0.01).CONCLUSIONS: The increase in syndecan-1 and protein C suggests that acute hypobaric hypoxia produces a minor degree of glycocalyx degradation and overall cellular damage. After hypoxia RHI rebounded to higher than baseline levels suggesting improved endothelial functionality.

AB - INTRODUCTION: Hypoxia is associated with increased capillary permeability. This study tested whether acute hypobaric hypoxia involves degradation of the endothelial glycocalyx.METHODS: We exposed 12 subjects to acute hypobaric hypoxia (equivalent to 4500 m for 2-4 h) and measured venous blood concentrations of biomarkers reflecting endothelial and glycocalyx degradation (catecholamines, syndecan-1, soluble CD40 ligand, protein C, soluble thrombomodulin, tissue-type plasminogen activators, histone-complexed DNA fragments, and nitrite/nitrate). Endothelial function was assessed by the hyperemic response to brachial artery occlusion by peripheral arterial tonometry.RESULTS: Compared with normoxic baseline levels, hypoxia increased concentrations of syndecan-1 from 22 (95% confidence interval: 17-27) to 25 (19-30) ng/ml (p < 0.02) and protein C from 76 (70-83)% to 81 (74-88)% (p < 0.02). Nitrite/nitrate decreased from 23 (18-27) μM at baseline to 19 (14-24) μM and 18 (14-21) μM in hypoxia and recovery, respectively (p < 0.05). Other biomarkers remained unchanged. The post-occlusion/pre-occlusion ratio (reactive hyperemia index, RHI) decreased from 1.80 (1.52-2.07) in normoxia to 1.62 (1.28-1.96) after 2-4 h of hypobaric hypoxia and thereafter increased to 2.43 (1.99-2.86) during normoxic recovery (p < 0.01).CONCLUSIONS: The increase in syndecan-1 and protein C suggests that acute hypobaric hypoxia produces a minor degree of glycocalyx degradation and overall cellular damage. After hypoxia RHI rebounded to higher than baseline levels suggesting improved endothelial functionality.

U2 - 10.3389/fphys.2014.00459

DO - 10.3389/fphys.2014.00459

M3 - Journal article

C2 - 25505423

VL - 5

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

M1 - 459

ER -

ID: 130288905