Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport.

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Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport. / Ai, Hua; Ralston, Evelyn; Lauritzen, Hans P M M; Galbo, Henrik; Ploug, Thorkil.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 285, Nr. 4, 2003, s. E836-44.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Ai, H, Ralston, E, Lauritzen, HPMM, Galbo, H & Ploug, T 2003, 'Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport.', American Journal of Physiology: Endocrinology and Metabolism, bind 285, nr. 4, s. E836-44. https://doi.org/10.1152/ajpendo.00238.2002

APA

Ai, H., Ralston, E., Lauritzen, H. P. M. M., Galbo, H., & Ploug, T. (2003). Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport. American Journal of Physiology: Endocrinology and Metabolism, 285(4), E836-44. https://doi.org/10.1152/ajpendo.00238.2002

Vancouver

Ai H, Ralston E, Lauritzen HPMM, Galbo H, Ploug T. Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport. American Journal of Physiology: Endocrinology and Metabolism. 2003;285(4):E836-44. https://doi.org/10.1152/ajpendo.00238.2002

Author

Ai, Hua ; Ralston, Evelyn ; Lauritzen, Hans P M M ; Galbo, Henrik ; Ploug, Thorkil. / Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport. I: American Journal of Physiology: Endocrinology and Metabolism. 2003 ; Bind 285, Nr. 4. s. E836-44.

Bibtex

@article{dcdb5320aca711ddb5e9000ea68e967b,
title = "Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport.",
abstract = "Insulin and muscle contractions stimulate glucose transport in skeletal muscle through a translocation of intracellular GLUT4 glucose transporters to the cell surface. Judged by immunofluorescence microscopy, part of the GLUT4 storage sites is associated with the extensive microtubule cytoskeleton found in all muscle fibers. Here, we test whether microtubules are required mediators of the effect of insulin and contractions. In three different incubated rat muscles with distinct fiber type composition, depolymerization of microtubules with colchicine for < or =8 h did not inhibit insulin- or contraction-stimulated 2-deoxyglucose transport or force production. On the contrary, colchicine at least partially prevented the approximately 30% decrease in insulin-stimulated transport that specifically developed during 8 h of incubation in soleus muscle but not in flexor digitorum brevis or epitrochlearis muscles. In contrast, nocodazole, another microtubule-disrupting drug, rapidly and dose dependently blocked insulin- and contraction-stimulated glucose transport. A similar discrepancy between colchicine and nocodazole was also found in their ability to block glucose transport in muscle giant {"}ghost{"} vesicles. This suggests that the ability of insulin and contractions to stimulate glucose transport in muscle does not require an intact microtubule network and that nocodazole inhibits glucose transport independently of its microtubule-disrupting effect.",
author = "Hua Ai and Evelyn Ralston and Lauritzen, {Hans P M M} and Henrik Galbo and Thorkil Ploug",
note = "Keywords: Animals; Colchicine; Deoxyglucose; Forelimb; Glucose Transporter Type 4; Hindlimb; Insulin; Male; Microtubules; Monosaccharide Transport Proteins; Muscle Contraction; Muscle Fibers; Muscle Proteins; Rats; Stress, Mechanical",
year = "2003",
doi = "10.1152/ajpendo.00238.2002",
language = "English",
volume = "285",
pages = "E836--44",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport.

AU - Ai, Hua

AU - Ralston, Evelyn

AU - Lauritzen, Hans P M M

AU - Galbo, Henrik

AU - Ploug, Thorkil

N1 - Keywords: Animals; Colchicine; Deoxyglucose; Forelimb; Glucose Transporter Type 4; Hindlimb; Insulin; Male; Microtubules; Monosaccharide Transport Proteins; Muscle Contraction; Muscle Fibers; Muscle Proteins; Rats; Stress, Mechanical

PY - 2003

Y1 - 2003

N2 - Insulin and muscle contractions stimulate glucose transport in skeletal muscle through a translocation of intracellular GLUT4 glucose transporters to the cell surface. Judged by immunofluorescence microscopy, part of the GLUT4 storage sites is associated with the extensive microtubule cytoskeleton found in all muscle fibers. Here, we test whether microtubules are required mediators of the effect of insulin and contractions. In three different incubated rat muscles with distinct fiber type composition, depolymerization of microtubules with colchicine for < or =8 h did not inhibit insulin- or contraction-stimulated 2-deoxyglucose transport or force production. On the contrary, colchicine at least partially prevented the approximately 30% decrease in insulin-stimulated transport that specifically developed during 8 h of incubation in soleus muscle but not in flexor digitorum brevis or epitrochlearis muscles. In contrast, nocodazole, another microtubule-disrupting drug, rapidly and dose dependently blocked insulin- and contraction-stimulated glucose transport. A similar discrepancy between colchicine and nocodazole was also found in their ability to block glucose transport in muscle giant "ghost" vesicles. This suggests that the ability of insulin and contractions to stimulate glucose transport in muscle does not require an intact microtubule network and that nocodazole inhibits glucose transport independently of its microtubule-disrupting effect.

AB - Insulin and muscle contractions stimulate glucose transport in skeletal muscle through a translocation of intracellular GLUT4 glucose transporters to the cell surface. Judged by immunofluorescence microscopy, part of the GLUT4 storage sites is associated with the extensive microtubule cytoskeleton found in all muscle fibers. Here, we test whether microtubules are required mediators of the effect of insulin and contractions. In three different incubated rat muscles with distinct fiber type composition, depolymerization of microtubules with colchicine for < or =8 h did not inhibit insulin- or contraction-stimulated 2-deoxyglucose transport or force production. On the contrary, colchicine at least partially prevented the approximately 30% decrease in insulin-stimulated transport that specifically developed during 8 h of incubation in soleus muscle but not in flexor digitorum brevis or epitrochlearis muscles. In contrast, nocodazole, another microtubule-disrupting drug, rapidly and dose dependently blocked insulin- and contraction-stimulated glucose transport. A similar discrepancy between colchicine and nocodazole was also found in their ability to block glucose transport in muscle giant "ghost" vesicles. This suggests that the ability of insulin and contractions to stimulate glucose transport in muscle does not require an intact microtubule network and that nocodazole inhibits glucose transport independently of its microtubule-disrupting effect.

U2 - 10.1152/ajpendo.00238.2002

DO - 10.1152/ajpendo.00238.2002

M3 - Journal article

C2 - 12746214

VL - 285

SP - E836-44

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 4

ER -

ID: 8462407