Demethoxycurcumin is a potent inhibitor of P-type ATPases from diverse kingdoms of life
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Demethoxycurcumin is a potent inhibitor of P-type ATPases from diverse kingdoms of life. / Dao, Trong Tuan; Sehgal, Pankaj; Thanh Tung, Truong; Møller, Jesper Vuust; Nielsen, John; Palmgren, Michael Broberg; Christensen, Søren Brøgger; Fuglsang, Anja Thoe.
I: P L o S One, Bind 11, Nr. 9, e0163260, 2016.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Demethoxycurcumin is a potent inhibitor of P-type ATPases from diverse kingdoms of life
AU - Dao, Trong Tuan
AU - Sehgal, Pankaj
AU - Thanh Tung, Truong
AU - Møller, Jesper Vuust
AU - Nielsen, John
AU - Palmgren, Michael Broberg
AU - Christensen, Søren Brøgger
AU - Fuglsang, Anja Thoe
PY - 2016
Y1 - 2016
N2 - P-type ATPases catalyze the active transport of cations and phospholipids across biological membranes. Members of this large family are involved in a range of fundamental cellular processes. To date, a substantial number of P-type ATPase inhibitors have been characterized, some of which are used as drugs. In this work a library of natural compounds was screened and we first identified curcuminoids as plasma membrane H+-ATPases inhibitors in plant and fungal cells. We also found that some of the commercial curcumins contain several curcuminoids. Three of these were purified and, among the curcuminoids, demethoxycurcumin was the most potent inhibitor of all tested P-type ATPases from fungal (Pma1p; H+-ATPase), plant (AHA2; H+-ATPase) and animal (SERCA; Ca2+-ATPase) cells. All three curcuminoids acted as non-competitive antagonist to ATP and hence may bind to a highly conserved allosteric site of these pumps. Future research on biological effects of commercial preparations of curcumin should consider the heterogeneity of the material.
AB - P-type ATPases catalyze the active transport of cations and phospholipids across biological membranes. Members of this large family are involved in a range of fundamental cellular processes. To date, a substantial number of P-type ATPase inhibitors have been characterized, some of which are used as drugs. In this work a library of natural compounds was screened and we first identified curcuminoids as plasma membrane H+-ATPases inhibitors in plant and fungal cells. We also found that some of the commercial curcumins contain several curcuminoids. Three of these were purified and, among the curcuminoids, demethoxycurcumin was the most potent inhibitor of all tested P-type ATPases from fungal (Pma1p; H+-ATPase), plant (AHA2; H+-ATPase) and animal (SERCA; Ca2+-ATPase) cells. All three curcuminoids acted as non-competitive antagonist to ATP and hence may bind to a highly conserved allosteric site of these pumps. Future research on biological effects of commercial preparations of curcumin should consider the heterogeneity of the material.
KW - Journal Article
U2 - 10.1371/journal.pone.0163260
DO - 10.1371/journal.pone.0163260
M3 - Journal article
C2 - 27644036
VL - 11
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
M1 - e0163260
ER -
ID: 166196698