Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training
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Dokumenter
- Deshmukh et al_Nature Communications_2021_Vol 12_e304
Forlagets udgivne version, 1,71 MB, PDF-dokument
Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 304 |
Tidsskrift | Nature Communications |
Vol/bind | 12 |
Antal sider | 15 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
CURIS 2021 NEXS 021
A correction to this has been published at: https://doi.org/10.1038/s41467-021-22015-4
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