Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid
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Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. / Olesen, Ann Sofie; Lohse, Louise; Dalgaard, Marlene Danner; Woźniakowski, Grzegorz; Belsham, Graham J; Bøtner, Anette; Rasmussen, Thomas Bruun.
I: Journal of Virological Methods, Bind 261, 11.2018, s. 14-16.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid
AU - Olesen, Ann Sofie
AU - Lohse, Louise
AU - Dalgaard, Marlene Danner
AU - Woźniakowski, Grzegorz
AU - Belsham, Graham J
AU - Bøtner, Anette
AU - Rasmussen, Thomas Bruun
N1 - Copyright © 2018 Elsevier B.V. All rights reserved.
PY - 2018/11
Y1 - 2018/11
N2 - African swine fever (ASF) is an important disease of domestic pigs and wild boar. The disease is caused by African swine fever virus (ASFV). In 2014, ASFV was introduced into Eastern Europe, and it has since then continued to spread within various Eastern European countries. Investigating differences in sequences between ASFV isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the Eastern European outbreaks have been reported. In this study, the complete genome sequence of a highly virulent ASFV was determined directly from erythrocyte-associated nucleic acids obtained from a pig experimentally infected with an isolate from Poland (ASFV POL/2015/Podlaskie). The sequence (ca. 189 kb) of this recent European ASFV showed 95 nt differences (99.95% identity) from the ASFV Georgia 2007/1 genome. The complete sequence of ASFV POL/2015/Podlaskie should assist further studies on the genetic diversity and evolution of the European ASFVs.
AB - African swine fever (ASF) is an important disease of domestic pigs and wild boar. The disease is caused by African swine fever virus (ASFV). In 2014, ASFV was introduced into Eastern Europe, and it has since then continued to spread within various Eastern European countries. Investigating differences in sequences between ASFV isolates may be a valuable tool to understand differences in virulence among them, however currently, no complete genome sequences of the viruses responsible for the Eastern European outbreaks have been reported. In this study, the complete genome sequence of a highly virulent ASFV was determined directly from erythrocyte-associated nucleic acids obtained from a pig experimentally infected with an isolate from Poland (ASFV POL/2015/Podlaskie). The sequence (ca. 189 kb) of this recent European ASFV showed 95 nt differences (99.95% identity) from the ASFV Georgia 2007/1 genome. The complete sequence of ASFV POL/2015/Podlaskie should assist further studies on the genetic diversity and evolution of the European ASFVs.
KW - African Swine Fever/virology
KW - African Swine Fever Virus/genetics
KW - Animals
KW - DNA, Viral/chemistry
KW - Erythrocytes/virology
KW - Genome, Viral
KW - Poland
KW - Sequence Analysis, DNA
KW - Swine
U2 - 10.1016/j.jviromet.2018.07.015
DO - 10.1016/j.jviromet.2018.07.015
M3 - Journal article
C2 - 30063908
VL - 261
SP - 14
EP - 16
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
ER -
ID: 257914400